@article { author = {Etrati Khosroshahi, Mohammad and Ghazanfari, Lida and Hasan-nejad, Zahra}, title = {Preliminary Results of Treating Cancerous Cells of Lung (QU-DB) by Hyperthermia using Diode Laser and Gold Coated Fe3O4/SiO2 Nano-Shells: An in-Vitro Assay}, journal = {Iranian Journal of Medical Physics}, volume = {9}, number = {4}, pages = {253-263}, year = {2012}, publisher = {Mashhad University of Medical Sciences}, issn = {2345-3672}, eissn = {2345-3672}, doi = {10.22038/ijmp.2013.469}, abstract = {Introduction In this study, we describe the results of controlled synthesis and application of gold coated Fe3O4/SiO2 nano-shells combined with the optical property of gold for enhancement of selective photothermal interaction with cancerous cells based on the surface plasmon resonance (SPR) Phenomena. Materials and Methods Magnetite Nano-Particles (MNPs) were prepared by means of co-precipitation. MNPs were modified with a thin layer of Silica using the Stober method. The amino-modified Fe3O4/SiO2 nano-shells were covered with gold colloids as a self-assembeled process. In-vitro assays were performed to determine the effect of apoptosis of the cells based on the cells morphological changes. Results The biologically inert nano-shells (85 nm) with a Magnetite/Silica core and a gold shell were optically activated. A successful laser-hyperthermia based on the thermal effect of surface plasmon resonance was performed using different gold concentrations. The thermal profile effects of laser power are presented as ideal cases of nanoshell-assisted photo-thermal therapy. The thermally-induced cell death has been shown to be dependent on NPs concentration and laser power density. The power densities of 157 and 184 W/cm2 caused complete cell death at the focal point of the laser beam.Cell damage was reduced by decreasing the power density of laser. Also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. Conclusion An optimized laser-(SPR) hyperthermia was obtained using a concentration of gold coated Fe3O4/SiO2 nano-shells concentration=0.1 mg/ml at intensity=157 W/cm2 at 60s.}, keywords = {Diode laser,Hyperthermia,Lung Cancerous Cell,Metallic Nano-Shells,Surface Plasmon Resonance}, url = {https://ijmp.mums.ac.ir/article_469.html}, eprint = {https://ijmp.mums.ac.ir/article_469_24aa12e410071bfae5cc84946e53f35b.pdf} }