Effect of Silver Nanoparticles on Improving the Efficacy of 5-Aminolevulinic Acid-Induced Photodynamic Therapy

Document Type: Original Paper

Authors

1 Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

2 Nuclear Medicine Research Center, Department of Modern Science and Technology, Mashhad University of Medical Sciences, Mashhad, Iran

3 mashhad university of medical science,medical physics department

4 Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

5 Medical Physics Dept., Mashhad University of Medical Sciences

Abstract

Introduction: The most important limitation of 5-aminolevulinic acid (5-ALA)-induced photodynamic therapy (PDT) is the efficacy of the cells in converting 5-ALA to protoporphyrin IX. The present study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) with the photosensitivity at the surface plasmon resonance wavelength on 5-ALA-mediated PDT.
Material and Methods: First of all, the toxicity of 5-ALA, AgNPs, and combined 5-ALA and AgNPs was evaluated on DFW cell line derived from melanoma. After choosing the optimal concentration, both pulsed and continuous light irradiations were conducted at different doses using a light‑emitting diode source in the groups receiving 5-ALA, AgNPs, as well as 5-ALA and AgNPs combination, and the controls. The cell survival was evaluated 24 h after irradiation using MTT assay.
Results: According to the results, light exposure did not significantly change cell survival in the absence or presence of AgNPs. However, light exposure in the presence of 5-ALA and AgNPs/5-ALA combination caused a significant reduction in the cell survival. The necessary light exposure to induce 50% cell death (ED50) in the presence of 5-ALA was 1280 mJ/cm2; however, this value was 280 mJ /cm2 in the presence of combined AgNPs and 5-ALA.
Conclusion: As the findings indicated, PDT had a higher efficacy in the presence of combined 5-ALA and AgNPs than in the sole 5-ALA presence. Nonetheless, further studies are required to evaluate the definitive mechanism of these findings. 

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