Assessment of in vitro radiosensitivity parameters of breast cancer cells following exposure to radiotherapy hospital-based facilities

Document Type: Original Paper

Authors

1 Physics Department, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

2 Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Department of Radiation Oncology, Shohada-e-Tajrish Hospital, Tehran, Iran

Abstract

Introduction: The aim of the present study was to assess the radiosensitivity parameters for SK-BR-3 (SKBR3) breast cancer cells that could be implemented in the cutting-edge treatment planning systems (TPS) for accelerated partial-breast irradiation (APBI).
Materials and Methods: The cell survival fraction and its relevant radiosensitivity coefficients, namely α and β, in linear-quadratic (LQ) formalism were evaluated for 6 MV X-rays and 60Co γ-rays using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. During the irradiation time, the medium temperature was kept at 4°C to prevent the repair of sublethal radiation damages over the exposure time and keep the survival fractions independent of the dose rate.
Results: Fitting the LQ model to experimental data, α, β, and α/β radiosensitivity parameters were obtained as 0.156±0.027 Gy-1, 0.026±0.007 Gy-2,and 6.0 Gy for 6 MV X-rays and 0.162±0.028 Gy-1, 0.028±0.007 Gy-2, and 5.8 Gy for 60Co gamma radiation, respectively. The average relative biological effectiveness (RBE) values were 0.91 and 0.96 for 6 MV X-rays and 60Co γ-rays, respectively. The derived LQ parameters were also compared with those previously obtained from in vitro studies for different breast cancer cell lines using various regimes, such as radiotherapy modality with different dose rates and delivered doses.
Conclusion: The results of this study provided essential constant values for α and β parameters. The data could be useful for the improvement of TPS to include the effect of different biological responses to radiation in APBI treatment plans. 

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  1. Smith BD, Arthur DW, Buchholz TA, Haffty BG, Hahn CA, Hardenbergh PH, et al. Accelerated partial breast irradiation consensus statement from the American Society for Radiation Oncology (ASTRO). Int J Radiat Oncol Biol Phys. 2009 Jul 15;74(4):987-1001.
  2. Cox JA, Swanson TA. Current modalities of accelerated partial breast irradiation. Nat Rev Clin Oncol. 2013 Jun 1;10(6):344-56.
  3. Tokatlı F, Dincer M. Breast-Conserving Therapy: Hypofractionated and Conventional Whole-Breast Irradiation and Accelerated Partial-Breast Irradiation. In: Aydiner A, Igci A, Soran A editors. Breast Disease: Management and Therapies. 1st ed. Switzerland: Springer International Publishing. 2016; 233-47.
  4. Ruiz de Almodóvar JM, Núñez MI, McMillan TJ, Olea N, Mort C, Villalobos M, et al. Initial radiation-induced DNA damage in human tumour cell lines: a correlation with intrinsic cellular radiosensitivity. Br J Cancer. 1994 Mar 1;69(3):457-62.
  5. Marthinsen AB, Gisetstad R, Danielsen S, Frengen J, Strickert T, Lundgren S. Relative biological effectiveness of photon energies used in brachytherapy and intraoperative radiotherapy techniques for two breast cancer cell lines. Acta Oncol. 2010 Nov 1;49(8):1261-8.
  6. Steel GG, Deacon JM, Duchesne GM, Horwich A, Kelland LR, Peacock JH. The dose-rate effect in human tumour cells. Radiother Oncol. 1987 Aug 1;9(4):299-310.
  7. Matthews JH, Meeker BE, Chapman JD. Response of human tumor cell lines in vitro to fractionated irradiation. Int J Radiat Oncol Biol Phys. 1989 Jan 1;16(1):133-8.
  8. Guerrero M, Li XA. Analysis of a large number of clinical studies for breast cancer radiotherapy: estimation of radiobiological parameters for treatment planning. Phys Med Biol. 2003 Sep 30;48(20):3307.
  9. Trialists' Group TS. The UK Standardisation of Breast Radiotherapy (START) Trial B of radiotherapy hypofractionation for treatment of early breast cancer: a randomised trial. The Lancet. 2008 Apr 4;371(9618):1098-107.
  10. Ray M, Tan AW, Mangunkusumo AE, Lim DK. Confocal Microscopy of Cornea. In: Ng E. Y. K, Acharya U. R, Rangayyan R. M, Suri J. S editors. Ophthalmological Imaging and Applications, 1st ed. Florida: CRC Press; 2014; 195-214.
  11. Buch K, Peters T, Nawroth T, Sänger M, Schmidberger H, Langguth P. Determination of cell survival after irradiation via clonogenic assay versus multiple MTT Assay-A comparative study. Radiat Oncol. 2012 Jan 3;7(1):1.
  12. Andreo P, Burns D T, Hohlfeld K, Huq M S, Kanai T, Laitano F, et al. Absorbed dose determination in external beam radiotherapy: An International Code of Practice for dosimetry based on standards of absorbed dose to water. 1st ed. Vienna: IAEA Technical Report Series No. 398; 2000.
  13. Scott BR, Hutt J, Lin Y, Padilla MT, Gott KM, Potter CA. Biological microdosimetry based on radiation cytotoxicity data. Radiat Prot Dosimetry. 2012 Aug 5;153(4):417-24.
  14. Kloss FR, Singh S, Hächl O, Rentenberger J, Auberger T, Kraft A, et al. BMP-2 immobilized on nanocrystalline diamond-coated titanium screws; demonstration of osteoinductive properties in irradiated bone. Head Neck. 2013 Feb 1;35(2):235-41.
  15. Calipel A, Lux AL, Guérin S, Lefaix JL, Laurent C, Bernaudin M, et al. Differential Radiosensitivity of Uveal Melanoma Cell Lines After X-rays or Carbon Ions Radiation. Invest Ophthalmol Vis Sci. 2015 May 1;56(5):3085-94.
  16. Dale, R G, Fowler J F. Radiation repair mechanisms. In: Dale R G, Jones B editors. Radiobiological modelling in radiation oncology, 1st ed. London, UK: British Inst of Radiology; 2007; 97-105.
  17. Menzel HG, Wambersie A, Pihet P. The clinical RBE and microdosimetric characterization of radiation quality in fast neutron therapy. Acta Oncol. 1994 Jan 1;33(3):251-9.
  18. Collins AR. The comet assay for DNA damage and repair: Mol Biotechnol. 2004 Mar 1;26(3):249.
  19. Collins AR, Dobson VL, Dusinská M, Kennedy G, Stĕtina R. The comet assay: what can it really tell us?. Mutat Res. 1997 Apr 29;375(2):183-93.
  20. Mozdarani H, Nasirian B, Haeri SA. In vivo gamma-rays induced initial DNA damage and the effect of famotidine in mouse leukocytes as assayed by the alkaline comet assay. J Radiat Res. 2007 Feb 14;48(2):129-34.
  21. Lechtman ES. A Monte Carlo-based model of gold nanoparticle radiosensitization. (Doctoral dissertation), University of Toronto; 2013. Retrieved from https://tspace.library.utoronto.ca/handle/1807/43632.
  22. Kelland LR, Steel GG. Dose-rate effects in the radiation response of four human tumour xenografts. Radiotherapy and oncology: Radiother Oncol. 1986 Nov 1;7(3):259-68.
  23. Hobbs RF, Howell RW, Song H, Baechler S, Sgouros G. Redefining relative biological effectiveness in the context of the EQDX formalism: Implications for alpha-particle emitter therapy. Radiat Res. 2013 Dec 30;181(1):90-8.
24. Matsuzaki H, Miyamoto T, Miyazawa Y, Okazumi S, Koide Y, Isono K. Biological Effects of Heavy Ion Beam on Human Breast Cancers. Breast Cancer. 1998 Jul 1;5(3):261