Investigated the effect of near inferared laser on Folliculogenesis cycle and compared with Colomiphene effects in vivo condition in rat’s ovary

Document Type: Conference Proceedings

Authors

1 Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Stem cell research Centre, Tabriz University of Medical Sciences, Tabriz, Iran

2 Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Stem cell research Centre, Tabriz University of Medical Sciences, Tabriz, Iran School of Medical Sciences, University of Aberdeen, Aberdeen, UK

Abstract

Introduction: Any disruption in Folliculogenesis cycle (FC) as a produces oocyte function, leads to ovulation diseases, like poly cystic ovarian syndrome (PCOS). Although, there have not been found any definite treatment for PCOS, there are some temporary methods to treat it. That’s including drug, surgery and also lasers as complementary. In addition, these methods not only don’t have short period treatment but also they increase the risk of woman’s breast cancer. In a world, this research investigated the photo-bio stimulation effect of low-level laser (180nm wavelength) on ovarian FC in the Rat’s ovary by Diode lasers as independent method.
Materials and Methods: In this study 21 female rats with the average age of 9 weeks in the 150-250 gr weight were used. The subjects were assigned to three groups: control (CT) clomiphene drug (D) and near infrared laser (NIRL), seven rats in each group. Afterwards, for 48 days in NIRL group, the rats received laser (5j/cm2) on estrous cycle. In D group, they received clomiphene drug (1µ/g in 1cc water solution). At the end, we anesthetized the animals and removed their ovary tissue. Henceforth, we investigated the lasers effects and compared with clomiphene. This protocol approved by Ethics and Research Committee of Tabriz university medical science (code number: TBZMED.REC.1394.238). Also this study’s results analyzed with statistical application as well.
Results: Laser is the main catalyst to increase different follicles (DF) producing to complement FC. The increase of DF significant compared with D and CT groups. It’s significant in D group to compare with CT. But it’s ratio in NIRL group was rather than D.
Conclusion: So, laser increase ovarian activity to produce DF. This result certainly can be used in future studies for finding a cure for negligent of ovary and treated diseases caused by ovarian negligence like PCOS. Finally, this study indicates that lasers can be used autonomously for treat, without any medical and surgical intervention.
Acknowledgments:
Hereby the authors express their gratitude to Eshrat khah and Musavi, Doctoral candidates, at the University of Tabriz for measuring hormone level; Abed Ellahi of the anatomy group, Tabriz University and Pedram Hamidi, master candidate at the Shahrood University, for designing rat restrainer and processing results by the SPSS software.

Keywords