Effects of Low Dose Gamma Ray on Some Hematological Parameters in Adult Rats

Document Type: Original Paper

Authors

1 Physics Dept., College of Science, Mosul University, Mosul

2 Department of Biophysics, College of Science, , University of Mosul, Mosul,IRAQ

3 Department of Physics, College of Science, University of Mosul, Mosul

Abstract

Introduction: The study aimed to find the low dose effect gamma ray on some hematological parameters in male albino rats obtained from the Mosul University, IRAQ, and also studied the radioprotective effect of black seed oil on the hematological parameters.
Material and Methods: The system is made of 241Am as gamma source .The rats were exposed to gamma radiation for limit period .Hematological investigations in which the red blood cells (RBC) also white blood cells (WBC) were found by the result of Neubauer haemocytometer method hemoglobin(Hb) concentration obtained due to cyanomethaemoglobin test. The packed cell volume (PCV) was determined using the micro-centrifuge method. Platelets were achieved.
Results: All hematological parameters found significantly decreased in all groups receiving daily dose of 6 mGy/h for 7h/day for 25, and 50 days compared with those in the control group. However group 2. Treated with 400 mg/kg wt /day for 25 days, showed a significant increase in the mentioned parameters.  Groups 3 and 4 were exposed to gamma ray in two stages(at a daily dose 6 mGy/h for 7h /day for 25 and 50 days) .Group 4 showed a significant enhancement  in hematological parameter when  treated  with  black seed oil , compared with group3 which had a significant decrease in these parameters compared with the control group.
Conclusion: The present work showed the function of black seed oil considered as an advanced material in decreasing the oxidant stress on hematological parameters againstgamma ray with limited dose.

Keywords

Main Subjects


  1. Soule B P, Hyodo, F, Matsumoto KI, Simone NL, Cook, JA, Krishna MC, et al.The chemistry and biology of nitroxide compounds. Free Radical Biology and Medicine, 2007; 42(11):1632-50.
  2. Ingrom, M. and T.A.C. Robert .Ionizing radiation ,In.Microbial Ecology of foods , Vol.1.pp46—69. ICMSF,Academic press ,New York .1980
  3. Allehayanim S. Monem S. Membrane solubilization and hemoglobin gelation as biomarker of radiation exposure to gamma-rays. Egyptain biophysics journal. 2003.;9. 234-44.
  4. Abdel-Salam OM, Hadajat I. Baiuomy AR. El-Shinawy S, Arbid MS.Exposure of rats to whole body gamma rays induces early alterations in biliary secretion. Turkish Journal of Medical Sciences. 2006; 36(5):263-9.  
  5. Hawas AM. Effect of low dose gamma rays on certain essential metals and oxidative stress in different rat organs.journal of radiation research and applied sciences.2013; 6(2):38-44.
  6. Padhye S, Banerjee S, Ahmad A, Mohammad R, Sarkar FH. From here to eternity-the secret of pharaohs: Therapeutic potential of black cumin seeds and beyond. Cancer therapy. 2008;6(b):495.
  7. Al-Janaby KA. The effect of aqueous extract of grassed on oxidative stress induced by hydrogen peroxide in male rat. Doctoral dissertation, Msc.Thesis.Tekrit University, IRAO. 2008.
  8. Orhon ZN, Uzal C, Kanter M,Erboga M, Demiroglu M. Protective effects of Nigella sativa on gamma radiation –induced jejunal mucosal damage in rats.Pathology-Research and Practice .2016;212(5):437-43.
  9. Steel RG, Torrie JH. Principle and procedure of statics. 2nd ed. New York: McGrow Hilibook Company .1980.
  10. Hofer M, Hoferova Z,Dušek L,Souček K. Gruzdev A. Hematological profile of untreated or ionizing radiation –exposed cyclooxygenase -2-deficient mice. Physiological research. 2017; 66(4):673.
  11. Yamamori T, Yasui H, Yamaxumi M, Wada Y, Nakamura Y, Nakamura H, et al .Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint . Free Radical Biology and Medicine .2012; 53(2):260-70.
  12. Daniak N. Hematologic consequences of exposure to ionizing radiation Experimental hematology 2012; 30(6):513-28.
  13. Ismael AH. Protective effect of Nigella sativa oilon reproductive system and some blood parameters of albino mice exposed to oxidative stress by hydrogen peroxide. PHD, Thesis in University of Mosul.IRAQ.2005.39-41.
  14. Fabiani R, Rosignoli P, De Bartolomeo A, Fuccelli R, Servili M, Montedoro GF, et al .Oxidative DNA damage is prevented by extracts of olive oil, hydroxytyrosol, and other olive phenolic coumpounds in human blood mononuclear cells and HL60 cells. The Journal of Nutrition. 2008; 138(8):1411-6. 
  15.  Thomas JP. Black seed oil cures many cancer according to numerous studies.Health Impact News.2014; 11(16):1-15.Avalable form: http://healthimpactnews,com.
  16. .El-Desouky W, Hanafi A, Abbas MM. Radioprotective effect of green tea and grope seed extracts mixture on gamma irradiation induced immune suppression in male albino rats. International journal of radiation biology .2017; (4):433-9.
  17.  Eshak MG, Osman HF. Role of Moringa oleifera leaves on biochemical and genetical alterations in irradiated male rats. Middle-East J Sci Res.2013; 16(10):1303-15.
  18.  Chew BP, Park JS.Carotenoid action on the immune response. The journal of nutrition. 2004;134(1):257-61