Document Type: Conference Proceedings
Department of Medical Biotechnology, School of Paramedical Sciences, Qazvin University of Medical Sciences, Iran.
Radiation Biology Research Center, Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Department of Medical Physics, Iran University of Medical Sciences, Tehran, Iran
Radiology & Radiotherapy department- Para Medical Faculty- Shahid Beheshti University of Medical sciences, Tehran, Iran.
Radiation Medicine Engineering Department, Shahid Beheshti University, Tehran, Iran
Introduction: The aim of this study was the evaluation of induced DNA damages of human prostate cancer cells, DU-145, treated with a combination of radiofrequency capacitive hyperthermia (HT) and teletherapy (EBRT) compared to a combination of teletherapy with high dose rate brachytherapy (BR).
Materials and Methods: DU-145 cells were cultured as spheroids in 300-micron diameter. Then the following treatments were conducted: a) EBRT at doses of either 2Gy or 4Gy of photon 15MV, b) HT for 30, 60, and 90 minutes’ duration at 43°C from a 13.56MHz radiofrequency capacitive heating device (Celsius TCS), c) BR with Ir-192 seed at doses of either 2Gy or 5.5Gy, d) The mentioned HT followed by EBRT (HT+EBRT) and e) EBRT followed by BR (EBRT+BR). Alkaline comet assay was performed to measure tail moment.
Results: The induced DNA damages of DU-145 cells treated by adding HT to EBRT compared with EBRT alone, showed a significant enhancement; 3.28 and 5.14 times respectively for 30 and 60 minutes HT. By plotting dose-response curves, we could find a range of doses, which create radiobiological iso-effect in HT+EBRT and EBRT+BR treatments.
Conclusion: This study suggests that about DNA damages of DU-145 cells, HT+EBRT could partly be considered as an alternative to EBRT+BR.