Radio-protective effects of Hesperidin against gamma radio-radio-protective effects of Hesperidin against gamma radiation induced histopathological changes in the liver of Sprague Dawely rats

Document Type: Conference Proceedings

Authors

1 MSC of Radiobiology & Radiation Protection, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran. E-mail address . Tel: +98 9104593624

2 Assistant Professor of Medical Physics, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

3 Associate Professor of Medical Physics, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

4 Professor of Pathology, Diagnostic Laboratory Sciences and Technology Research Center, Basic Sciences in Infection Diseases Research Center, Center of Comparative & Experimental Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Abstract

Introduction: Despite the extensive use of ionizing radiation in diagnosis and therapeutic procedures, development of radio-protector agents is important in modulating normal tissue damages. Hesperidin(HES) is a Flavanone glycoside, belonging to the Flavonoid family in human diet abundantly and noted for its many beneficial effects. The aim of present study was investigated to the radio-protective effects of Hesperidin on radiation-induced hepatotoxicity in male Sprague Dawely rat.
Materials and Methods: 40 male Sprague Dawley rats were selected randomly and divided into 8groups: control, Hesperidin (100 mg/kg body weight, orally, 7 days), whole body gamma radiation with 2 Gy and 8 Gy, pre-administrated with 50 and 100 mg/kg body weight of Hesperidin for 7 days before irradiation with 2Gy and 8 Gy, respectively. Histopathological evaluation was done 24 h after radiation.
Results: Administration of Hesperidin (50 and 100 mg/kg b.w, orally, 7 days) before 2 Gy of gamma radiation led to remove inflammatory mononuclear cells in the portal space but there was still dilation of central veins while hesperidin at a dose of 100 mg/kg before 2 Gy gamma radiation was observed normal liver structure. Microscopic findings in the groups in the groups receiving two doses of Hesperidin (50 and 100 mg/kg b.w , orally, 7 days) before 8 Gy of gamma radiation was similar , in this way that extreme dilation of central veins to be seen but there was no capillarization.
Conclusion: Based on the finding in this study, it shows that Hesperidin would ameliorate the radiation-induced damage. Therefore, HES may be offered as suitable radio-protector in radiotherapy patients, radiation workers and public.

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