Document Type: Conference Proceedings
Department of Radiology Technology, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Science, Tehran, Iran
Department of Medical Physics, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran
Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Introduction: Head-and-neck cancer is the sixth most common cancer worldwide with the number of cases consistently increasing in developing countries. Successful development of effective, safe and cost effective nanoprobes for head-and-neck cancer targeting imaging is a big challenge. This study is aimed to develop cysteamine-folate conjugated gold nanoparticles (F-Cys-AuNPs) as a new contrast agent for targeted X-ray computed tomography imaging (CT) of head-and-neck cancer cells. In the other hand, this study is aimed to evaluation of effect of incubation times of multifunctional nanoparticles on cancer cells by CT.
Materials and Methods: The formed multifunctional F-Cys-AuNPs were characterized via different techniques. Transmission electron microscopy (TEM) was performed to investigate morphology and size of the GNPs. The concentrations of GNPs in μg/ml were measured by the inductively coupled plasma optical emission spectrometry (ICP-OES). The cytocompatibility of the F-Cys-AuNPs were assessed by MTT and colony assays. Targeted ability of F-Cys-AuNPs was evaluated in head-and-neck cancer in different incubation times.
Results: F-Cys-AuNPs nanoprobes with an Au core size of ~15 nm exhibited good biocompatibility, and could target actively the cancer cells. Our results indicated that a greater mass concentration in all the nanoparticles and increasing incubation times lead to greater X-ray attenuation and totally in clinic lead to payload the delivery of a larger mass in the site of interest, therefore, the contrast will be enhanced.
Conclusion: This data can be also considered for the application of gold nanostructures in radiation dose enhancement where nanoparticles with high X-ray attenuation are applied. The X-ray attenuation and the ease of the surface functionalization, make targeted GNPs promising multifunctional probes for simultaneous imaging and drug or gene delivery applications.