Enhancement of radio sensitization by gold-silica shell- core nanoparticle in MCF7 breast cancer cells

Document Type: Conference Proceedings

Authors

1 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

2 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Science, Tabriz, Iran

3 Department of Medical Biotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran

4 Tabriz Valiasr Hospital, Department of Radiation Oncology, Tabriz, Iran

5 Tuberculosis and Lung Disease Research Center of Tabriz, Tabriz University of Medical Sciences Department of Chemical Engineering, Northeastern University, Boston, MA, USA

6 Faculty of Advanced Medical Sciences, Tabriz University of Medical Science, Tabriz, Iran

7 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Department of Medical Physic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran Imam Reza University Hospital, Radiotherapy Department, Tabriz University of Medical sciences, Tabriz, Iran

Abstract

 
Introduction: In the present study, we investigated the role of gold-silica shell-core nanoparticle in megavoltage irradiation of MCF7 breast cancer cells.
 
Materials and Methods: gold-silicon oxide shell-core nanoparticles (NPs) were obtained by conjugation of gold nanoparticle with amine or thiol functionalized silica nanoparticles (AuN@SiO2 and AuS@SiO2). The structure of each nanoparticle was fully characterized by FTIR and TEM. The NPs treated cells were irradiated by using 6 and 18 MV x-rays. MTT and Brdu assay, DAPI staining and cell cycle were used to evaluate efficiency of nanoparticle in radiation therapy.
Results: The average size of AuN@SiO2 and AuS@SiO2 NPs was 23.79±7.04 and 31.28±7.37 nm, respectively. The Brdu findings revealed significantly more death in AuN@SiO2 (amine) treated and exposed cells compared to AuS@SiO2 (thiol) treated cells. (p<0.05). Cell cycle study show that presence of NPs enhanced the effect of radiotherapy, in particular, highest destructive effects were observed at the radiation dose of 8 Gy using energy 18 MV.
 
Conclusion: It was concluded that our findings may open the possibilities for cooperative radiotherapy and gold-silica shell-core nanoparticle by increasing cytotoxicity in MCF7 cells and decreased side effect from single modality radiotherapy.

Keywords