Correlation between the Energy Density of Ultraviolet Radiation and Skin Thickening In an Animal Mouse Model: Induction of Trichoepithelioma in a Mouse Model

Document Type : Original Paper

Authors

1 Department of Medical Physics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2 Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

3 Department of Pathology, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran

Abstract

Introduction: Exposure to ultraviolet (UV) radiation causes oxidative damage and cancer in the epidermis. The thickening of the skin layer seems to be correlated with carcinogenicity. The present study aimed to induce trichoepithelioma, a rare benign skin lesion, in an animal model and investigate the relationship between the radiation dose of UV waves and the thickening of skin layers resulting from high-frequency ultrasound images.
Material and Methods: To investigate skin damage process, 25 C57BL6 mice were irradiated with Ultraviolet B-rays (UVB) (5 times a week for 9 weeks) with an energy density of 135, 270, 405, 540, 675, 810, 945, 1080, and 1215 J/m2, from the first week to the ninth week, respectively. The thickness of the skin layer was weekly measured by ultrasound images. The correlation between the thickness of the skin layer and the radiation energy density was analyzed by Pearson correlation analysis.
Results: The thickness of the skin layer demonstrated a significant increase in the 7th week of exposure during the injury process due to UV radiation, as compared to zero-day (P˂0.05). Furthermore, it showed a 38 % increase in the 7th week. The obtained results illustrated a significant correlation coefficient of more than 0.97 between the thickness of the skin layer and the energy density of UV radiation. Microscopic sections in the long-term UV-irradiated group confirmed trichoepithelioma.
Conclusion: As evidenced by the obtained results, prolonged irradiation for 9 weeks induced an animal model of trichoepithelioma.

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References

  1. Krutmann J, Schikowski T, Huls A, Vierkotter A, Grether-Beck S. Environmentally induced (extrinsic) skin aging. Hautarzt. 2016; 67(2): 99-102.
  2. Nishisgori C. Current concept of photocarcinogenesis. Photochem Photobiol Sci. 2015; 14(9): 1713-21.
  3. Kammeyer A, Luiten RM. Oxidation events and skin aging. Ageing Res Rev. 2015; 21: 16-29.
  4. Christensen L, Suggs A, Baron E. Ultraviolet photobiology in dermatology. Adv Exp Med Biol. 2017; 996: 89-104.
  5. Salmaninejad A, Kangari P, Shakoori A. Oxidative stress: Development and progression of breast cancer: Review article. Tehran Univ Med J. 2017; 75(1): 1-9.
  6. Liguori I, Russo G, Curcio F, Bulli G, Aran L, Della-Morte D, et al. Oxidative stress, aging, and diseases. Clin Interv Aging. 2018; 13: 757-72.
  7. Kabir S, Schmults C, Ruiz E. A review of cutaneous squamous cell carcinoma epidemiology, diagnosis, and management. Int J Cancer Manag. 2018; 11(1): e60846.
  8. Sharma MR, Werth B, Werth VP. Animal models of acute photodamage: Comparisons of anatomic, cellular and molecular responses in C57BL/6J, SKH1 and Balb/c mice. Photochem Photobiol. 2011; 87(3): 690-8.
  9. D'Orazio J, Jarrett S, Amaro-Ortiz A, Scott T. UV radiation and the skin. Int J Mol Sci. 2013; 14(6): 12222-48.
  10. Chen WJ, Chang YY, Shen SC, Tzeng YL, Lee HC, Yang CH, et al. In vivo detection of UV-induced acute skin effects using optical coherence tomography. Biomed Opt Express. 2018; 9(9): 4235-45.
  11. Ahmadi Ashtiani HR, Hekmat Nazemi N, Rezazadeh S, Gholamhoseini B, Baghaei M, Ehsani AH, et al. The evaluation of anti-UV effect of silymarin cream based on clinical and pathological findings. Tehran Uni Med J. 2010; 68(6): 321-9.
  12. Wang XF, Huang YF, Wang L, Xu LQ, Yu XT, Liu YH, et al. Photo-protective activity of pogostone against UV-induced skin premature aging in mice. Exp Gerontol. 2016; 77: 76-86.
  13. Scott TL, Christian PA, Kesler MV, Donohue KM, Shelton B, Wakamatsu K, et al. Pigment-independent cAMP-mediated epidermal thickening protects against cutaneous UV injury by keratinocyte proliferation. Exp Dermatol. 2012; 21(10): 771-7.
  14. Raad H, Serrano-Sanchez M, Harfouche G, Mahfouf W, Bortolotto D, Bergeron V, et al. NADPH Oxidase-1 plays a key role in keratinocyte responses to UV radiation and UVB-induced skin carcinogenesis. J Investe Dermatol. 2017; 137(6): 1311-21.
  15. Moon S, Youn J, Kim J. The effect of ultraviolet-B exposure scheduling on the photodamage of hairless mouse skin. Photodermatol Photoimmunol Photomed. 2000; 16: 74-7.

 

Iranian Journal of Medical Physics
Volume 18, Issue 2
March and April 2021
Pages 106-110

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History

  • Receive Date: 14 November 2019
  • Revise Date: 08 March 2020
  • Accept Date: 16 March 2020

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