Introducing an Optimized Method for Obtaining X-ray Diffraction Patterns of Biological Tissues

Document Type: Original Paper

Authors

1 Medical Physic Dept., Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Medical Physic Dept., Tehran University of Medical Sciences, Tehran, Iran

3 Radiology Technology Dept., Tehran University of Medical Sciences, Tehran, Iran

Abstract

Introduction
Individual X-Ray diffraction patterns of biological tissues are obtained via interference of coherent scattering with their electrons. Many scientists have distinguished normal and cancerous breast tissue, bone density, and urinary stone types using the X-Ray diffraction patterns resulting from coherent scattering. The goal of this study was to introduce an optimized method for obtaining X-ray diffraction patterns of different types from biological tissues.
Materials and Methods
A special tool constituting primary and scatter collimators as well as a sample holder was designed and built. All measurements were done using an X-ray tube, the above-mentioned tool, and a semiconductor detector (HPGe). The X-ray diffraction patterns of some tissue-equivalent materials (acrylic, polyethylene, nylon, and calcium carbonate) and biological tissues (adipose, muscle, and bone) were obtained.
Results
The corresponding peak positions for adipose, muscle, bone, acrylic, polyethylene, nylon, and calcium carbonate in corresponding X-ray diffraction patterns are located in 1.1±0.055 nm-1, 1.41±0.072, 1.6±0.08 nm-1, 0.8±0.04 nm-1, 1.03±0.051 nm-1, 1.22±0.061 nm-1, and 1.7 ± 0.085 nm-1, respectively.
Conclusion
The X-ray diffraction patterns obtained in this study were in good agreement relative to previous measurements in terms of peak position. This study introduces a useful setup for extraction of X-ray diffraction patterns from different biological tissues.

Keywords

Main Subjects


  1. Chaparian A, Oghabian MA, Changizi V, Farquharson MJ. The optimization of an energy dispersive x-ray diffraction system for potential clinical application. Appl Radiat Isot. 2010 Dec;68(12):2237-45.
  2. LeClair RJ, Boileau MM, Wang Y. A semianalytic model to extract differential linear scattering coefficient of breast tissue from energy dispersive x-ray diffraction measurements. Med Phys. 2006 Apr;33(4):959-67.
  3. Batchelar DL, Davidson MT, Dabrowski W, Cunningham IA. Bone-composition imaging using Coherent-scatter computed tomography: Assessing bone health beyond bone mineral density. Med Phys. 2006 Apr;33(4):904-15.
  4. Davidson MT, Batchelar DL, Chew BH, Denstedt JD, Cunningham IA. Establishing Composition and Structure of Intact Urinary Calculi by X-Ray Coherent Scatter for Clinical Laboratory Investigations. J Urol. 2006 Jun;175(6):2336-40.
  5. Royle GJ, Speller RD. Quantitative X-ray diffraction analysis of bone and marrow volumes in excised femoral head samples. Phys Med Biol. 1995 Sep;40(9):1487-98.
  6. Kidane G, Speller RD, Royle GJ, Hanby AM. X-ray scatter signatures for normal and neoplastic breast tissues. Phys Med Biol. 1999 Jul;44(7):1791-802.
  7. Poletti ME, Gonçalves D, Mazzaro I. X-ray scattering from human breast tissues and breast-equivalent materials. Phys Med Biol. 2002 Jan 7;47(1):47-63.
  8. Changizi V, Oghabian MA, Speller R, Sarkar S, Kheradmand AA. Application of small angle x-ray scattering (SAXS) for differentiation between normal and cancerous breast tissue. Int J Med Sci. 2005;2(3):118-21. Epub 2005 Jul 5.
  9. Changizi V, Kheradmand AA, Oghabian MA. Application of small angle x-ray scattering (SAXS) for differentiation among breast tumors. J Med Phys. 2008 Jan;33(1):19-23.
  10. Narten AH. X-ray diffraction data on liquid water in the temperature range 4 °C–200 °C. ORNL 1970 Report No. 4578.
  11. Theodorakou C, Farquharson MJ. Human soft tissue analysis using x-ray or gamma-ray techniques Phys Med Biol. 2008 Jun 7;53(11):R111-49. Epub 2008 May 1.
  12. Tartari A, Taibi A, Bonifazz C, Baraldi C. Updating of form factor tabulations for Coherent scattering of photons in tissues. Phys Med Biol. 2002 Jan 7;47(1):163-75.
  13. Chaparian A, Oghabian MA, Changizi V. Acquiring molecular interference functions of X-ray Coherent scattering for breast tissues by combination of simulation and experimental methods. Iran J Radiat Res. 2009;7(2):113-7.