Molecular Effects of Atmospheric Pressure Plasma Jet on the Double-Stranded DNA

Document Type: Original Paper

Authors

1 Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran

2 Department of Atomic and Molecular Physics, Faculty of Basic Sciences, University of Mazandaran

Abstract

Introduction
The aim of this study was toinvestigate the sterilization potential of atmospheric pressure plasma jet (APPJ) and interactions of this technology with double-stranded DNA using the polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP) techniques.
Materials and Methods
The plasma jet was produced through a high voltage sinusoidal power supplyusing a mixture of argon and oxygen gases with theflow rate of 1 L/min. Escherichia coli cells and double-stranded DNA (dsDNA) fragments were amplified by T7 universal primer through the PCR technique and treated with argon/oxygen APPJ at different exposure times. The data were analyzed by the agarose and polyacrylamide gel electrophoresis, SSCP and renewed PCR techniques.
Results
According to the results of the study, the APPJ could serve as an effective instrument for sterilization at > 30 sec discharge. The destruction of DNA was detectable by different techniques after 120 sec from APPJ discharge.
Conclusion
Our findings revealed that the active species of plasma can lead to cell death. These species may break or nick the dsDNA, exchange DNA nucleotides, and lead to transition and transversion mutations. These mutagenesis effects of APPJ might be the reason of microorganism cell death after the treatment in addition to other destructive effects of APPJ on macromolecules. 

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