TY - JOUR ID - 17590 TI - Time-Dependent Induction of the Nucleotide Excision Repair Gene XPA and RAD51 in Homologous Recombination in Human Lymphocytes Exposed to Low Doses of Ionizing Radiation JO - Iranian Journal of Medical Physics JA - IJMP LA - en SN - AU - Bahreyni Toossi, Mohammad Taghi AU - Khademi, Sara AU - Azimian, Hosein AD - Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Radiology Technology, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran Y1 - 2022 PY - 2022 VL - 19 IS - 1 SP - 9 EP - 13 KW - DNA damage KW - Gene expression KW - Ionizing radiation KW - XPA KW - RAD51 DO - 10.22038/ijmp.2021.53719.1884 N2 - Introduction: The aim of the present study was to understand the effect of low-doses of ionizing radiation (LDIR) on repair genes expression in blood samples that were taken from healthy donors. The next purpose was to examine the time-effect on the modified gene expression caused by low-doses of ionizing radiation.Material and Methods: The RNA of peripheral blood lymphocytes (PBLs) taken from four healthy donors was isolated at different time points after exposure including 4, 24, 48, 72, and 168 hours and then cDNA was synthesized. Modification of XPA and RAD51 expression levels due to LDIR (2, 5, 10 cGy) were evaluated by relative quantitative reverse transcription-polymerase chain reaction.Results: Significant up-regulation of both repair genes was observed at the 4 and 168 h following to 10 cGy.  Also, this dose could increase expression levels of RAD51 at 48 and 72 h after radiation. For lower doses at 5 cGy, only XPA levels were significantly up-regulated after 168 h. A significant regression was found between the XPA levels and the dose, at 168 h after irradiation to PBLs that can represent a new potential biomarker for biological dosimetry purposes.Conclusion: The results of this study could support the hypothetical role of the different DNA repair pathways in response to LDIR. This led us to propose a molecular biodosimetry method for ionizing radiation in the range of LDIR. UR - https://ijmp.mums.ac.ir/article_17590.html L1 - https://ijmp.mums.ac.ir/article_17590_b0cac4c91f261a2b1fb7c25aac755e1f.pdf ER -