Iranian Journal of Medical Physics

Iranian Journal of Medical Physics

Effect of Silver Nanoparticles on Improving the Efficacy of 5-Aminolevulinic Acid-Induced Photodynamic Therapy

Document Type : Original Paper

Authors
Samaneh Veisi Yazdi 1
Majid Darroudi 2
Armin Imanparast 3
Fatemeh Hataminia 4
Ameneh Sazgarnia 5
1 Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
2 Nuclear Medicine Research Center, Department of Modern Science and Technology, Mashhad University of Medical Sciences, Mashhad, Iran
3 mashhad university of medical science,medical physics department
4 Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
5 Medical Physics Dept., Mashhad University of Medical Sciences
10.22038/ijmp.2017.27921.1301
Abstract
Introduction: The most important limitation of 5-aminolevulinic acid (5-ALA)-induced photodynamic therapy (PDT) is the efficacy of the cells in converting 5-ALA to protoporphyrin IX. The present study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) with the photosensitivity at the surface plasmon resonance wavelength on 5-ALA-mediated PDT.
Material and Methods: First of all, the toxicity of 5-ALA, AgNPs, and combined 5-ALA and AgNPs was evaluated on DFW cell line derived from melanoma. After choosing the optimal concentration, both pulsed and continuous light irradiations were conducted at different doses using a light‑emitting diode source in the groups receiving 5-ALA, AgNPs, as well as 5-ALA and AgNPs combination, and the controls. The cell survival was evaluated 24 h after irradiation using MTT assay.
Results: According to the results, light exposure did not significantly change cell survival in the absence or presence of AgNPs. However, light exposure in the presence of 5-ALA and AgNPs/5-ALA combination caused a significant reduction in the cell survival. The necessary light exposure to induce 50% cell death (ED50) in the presence of 5-ALA was 1280 mJ/cm2; however, this value was 280 mJ /cm2 in the presence of combined AgNPs and 5-ALA.
Conclusion: As the findings indicated, PDT had a higher efficacy in the presence of combined 5-ALA and AgNPs than in the sole 5-ALA presence. Nonetheless, further studies are required to evaluate the definitive mechanism of these findings. 
Keywords
Photodynamic Therapy
Nanoparticle
5-Aminolevolinic Acid
DFW Cell Line
Protoporphyrin IX
Subjects
  1. El Kassas HY, Attia AA. Bactericidal application and cytotoxic activity of biosynthesized silver nanoparticles with an extract of the red seaweed Pterocladiella capillacea on the HepG2 cell line. Asian Pac J Cancer Prev. 2014;15(3):1299-306.
  2. Mousavi S, DONG ZX, Sharifi A, Hersey P. Study of rose bengal-induced cell death in melanoma cells in the absence of light. 2006.
  3. Agostinis P, Berg K, Cengel KA, Foster TH, Girotti AW, Gollnick SO, et al. Photodynamic therapy of cancer: an update. CA: a cancer journal for clinicians. 2011;61(4):250-81.
  4. Yoo J-O, Ha K-S. 4 New Insights into the Mechanisms for Photodynamic Therapy-Induced Cancer Cell Death. International review of cell and molecular biology. 2012;295:139.
  5. Wachowska M, Muchowicz A, Firczuk M, Gabrysiak M, Winiarska M, Wańczyk M, et al. Aminolevulinic acid (ALA) as a prodrug in photodynamic therapy of cancer. Molecules. 2011;16(5):4140.
  6. Inoue H, Kajimoto Y, Shibata M-A, Miyoshi N, Ogawa N, Miyatake S-I, et al. Massive apoptotic cell death of human glioma cells via a mitochondrial pathway following 5-aminolevulinic acid-mediated photodynamic therapy. Journal of neuro-oncology. 2007;83(3):223-31.
  7. Khaing Oo MK, Yang X, Du H, Wang H. 5-aminolevulinic acid-conjugated gold nanoparticles for photodynamic therapy of cancer. 2008.
  8. Yang X, Palasuberniam P, Kraus D, Chen B. Aminolevulinic acid-based tumor detection and therapy: molecular mechanisms and strategies for enhancement. International journal of molecular sciences. 2015;16(10):25865-80.
  9. Khorsandi KH, fateh M, Hosainzadeh R. Applications and Advantages of Nanostructures in Photodynamic Therapy. Journal of Laser in Medicine. 2015,12(3):34-44.
  10. Mfouo-Tynga I, ElHussein A, AbdelHarith M, Abrahamse H. Photodynamic ability of silver nanoparticles in inducing cytotoxic effects in breast and lung cancer cell lines. Int J Nanomed. 2014;9(1):3771-80.
  11. Wei L, Lu J, Xu H, Patel A, Chen Z-S, Chen G. Silver nanoparticles: synthesis, properties, and therapeutic applications. Drug Discovery Today. 2015;20(5):595-601.
  12. Shahverdi AR, Fakhimi A, Shahverdi HR, Minaian S. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomedicine: Nanotechnology, Biology and Medicine. 2007;3(2):168-71.
  13. Amendola V, Bakr OM, Stellacci F. A study of the surface plasmon resonance of silver nanoparticles by the discrete dipole approximation method: effect of shape, size, structure, and assembly. Plasmonics. 2010;5(1):85-97.
  14. Foldbjerg R, Dang DA, Autrup H. Cytotoxicity and genotoxicity of silver nanoparticles in the human lung cancer cell line, A549. Archives of toxicology. 2011;85(7):743-50.
  15. Lalitha P. Apoptotic efficacy of biogenic silver nanoparticles on human breast cancer MCF-7 cell lines. Progress in biomaterials. 2015;4(2-4):113-21.
  16. Franco-Molina MA, Mendoza-Gamboa E, Sierra-Rivera CA, Gómez-Flores RA, Zapata-Benavides P, Castillo-Tello P, et al. Antitumor activity of colloidal silver on MCF-7 human breast cancer cells. Journal of Experimental & Clinical Cancer Research. 2010;29(1):148.
  17. Liu CJ, Wang CH, Chien CC, Yang TY, Chen ST, Leng WH, et al. Enhanced x-ray irradiation-induced cancer cell damage by gold nanoparticles treated by a new synthesis method of polyethylene glycol modification. Nanotechnology. 2008;19(29):295104.
  18. Yu H, Zhang Q, Liu H, Dahl M, Joo JB, Li N, et al. Thermal synthesis of silver nanoplates revisited: a modified photochemical process. ACS nano. 2014;8(10):10252-61.
  19. Yadegari-Dehkordi S, Sadeghi H R, Soudmand S, Shandiz F H, Sazgarnia A. Enhanced cytotoxicity of silver nanoparticles by the use of electrical pulses. Journal of Isfahan Medical School. 2014, 32(289):289.
  20. Kim CH, Chung C-W, Choi KH, Yoo J-J, Kim DH, Jeong Y-I, et al. Effect of 5-aminolevulinic acid-based photodynamic therapy via reactive oxygen species in human cholangiocarcinoma cells. International journal of nanomedicine. 2011;6:1357.

 

Iranian Journal of Medical Physics
Volume 15, Issue 4 - Serial Number 4
Autumn 2018
Pages 308-314

  • Receive Date 07 December 2017
  • Revise Date 27 December 2017
  • Accept Date 30 December 2017