In Vitro Investigation into Plasmonic Photothermal Effect of Hollow Gold Nanoshell Irradiated with Incoherent Light

Document Type : Original Paper


1 mashhad university of medical science,medical physics department

2 Assistant Professor of Organic Chemistry, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran.

3 Medical Physics Dept., Mashhad University of Medical Sciences


Introduction: Hollow gold nanoshells (HAuNS) are one of the most attractive nanostructures for biomedical applications due to their interesting physicochemical properties. This study sought to evaluate the plasmonic photothermal effect of HAuNS irradiated with incoherent light on melanoma cell line.
Materials and Methods: After the synthesis of nanostructures, the temperature changes of HAuNS and polyethylene glycol stabilized HAuNS (HAuNS-PEG) were evaluated at different irradiation dose levels. After determining the potential cytotoxicity of the agents, the DFW cells were irradiated by incoherent light with and without the nanostructures at different exposure doses with two spectral bands of 670±25 nm and 730±25 nm. Finally, the rate of the cell survival was determined by 1-Methyltetrazole-5-Thiol assay 24 h after irradiating.
Results: The HAuNS, HAuNS-PEG, and light exposure did not have any significant effect on the cell survival, individually. Stabilizing with PEG led to an increase in size and decreased their polydispersity index, zeta potential, and conductivity. The slopes of temperature and cell death caused by 730 nm were greater than 670 nm when the cells were irradiated in the presence of nanostructures. These changes became more significant with increasing the dose of exposure and HAuNS (or HAuNS-PEG) concentration. The lowest cell survival occurred in the concentration of 250 μg/ml of nanostructures and an exposure dose of 9 min (P<0.05).
Conclusion: the HAuNS-PEG significantly reduced its conductivity that leads to decreased plasmonic photothermal effect. Additionally, using an incoherent light with more spectral overlap for irradiating the nanostructures increased its thermal effects.


Main Subjects

  1. References


    1. Ban Q, Bai T, Duan X, Kong J. Noninvasive photothermal cancer therapy nanoplatforms via integrating nanomaterials and functional polymers. Biomaterials Science. 2017;5:190-210.
    2. Zou L, Wang H, He B, Zeng L, Tan T, Cao H, et al. Current Approaches of Photothermal Therapy in Treating Cancer Metastasis with Nanotherapeutics. Theranostics. 2016;6:762-72.
    3. Hwang S, Nam J, Jung S, Song J, Doh H, Kim S. Gold nanoparticle-mediated photothermal therapy: current status and future perspective. Nanomedicine. 2014;9:2003-22.
    4. Huang X, El-Sayed MA. Plasmonic photo-thermal therapy (PPTT). Alexandria Journal of Medicine. 2011;47:1-9.
    5. Choi J, Yang J, Jang E, Suh JS, Huh YM, Lee K, et al. Gold nanostructures as photothermal therapy agent for cancer. Anti-cancer agents in medicinal chemistry. 2011;11:953-64.
    6. Kafshdooz L, Kafshdooz T, Razban Z, Akbarzadeh A. The application of gold nanoparticles as a promising therapeutic approach in breast and ovarian cancer. Artificial cells, nanomedicine, and biotechnology. 2016;44:1222-7.
    7. Nicol JR, Dixon D, Coulter JA. Gold nanoparticle surface functionalization: a necessary requirement in the development of novel nanotherapeutics. Nanomedicine (London, England). 2015;10:1315-26.
    8. Huang X, El-Sayed MA. Gold nanoparticles: Optical properties and implementations in cancer diagnosis and photothermal therapy. Journal of Advanced Research. 2010;1:13-28.
    9. Lal S, Clare SE, Halas NJ. Nanoshell-enabled photothermal cancer therapy: impending clinical impact. Accounts of chemical research. 2008;41:1842-51.
    10. Singhana B, Slattery P, Chen A, Wallace M, Melancon MP. Light-activatable gold nanoshells for drug delivery applications. AAPS PharmSciTech. 2014;15:741-52.
    11. Chen J, Liang H, Lin L, Guo Z, Sun P, Chen M, et al. Gold-Nanorods-Based Gene Carriers with the Capability of Photoacoustic Imaging and Photothermal Therapy. ACS applied materials & interfaces. 2016;8:31558-66.
    12. Jiang T, Zhang B, Shen S, Tuo Y, Luo Z, Hu Y, et al. Tumor Microenvironment Modulation by Cyclopamine Improved Photothermal Therapy of Biomimetic Gold Nanorods for Pancreatic Ductal Adenocarcinomas. ACS applied materials & interfaces. 2017;9:31497-508.
    13. Xia Y, Li W, Cobley CM, Chen J, Xia X, Zhang Q, et al. Gold nanocages: from synthesis to theranostic applications. Accounts of chemical research. 2011;44:914-24.
    14. Patrick WA, Wagner HB. Method for Complete Deoxygenation of Water. Analytical Chemistry. 1949;21:752-3.
    15. Schwartzberg AM, Olson TY, Talley CE, Zhang JZ. Synthesis, Characterization, and Tunable Optical Properties of Hollow Gold Nanospheres. The Journal of Physical Chemistry B. 2006;110:19935-44.
    16. Abbasi S, Servatkhah M, Keshtkar MM. Advantages of using gold hollow nanoshells in cancer photothermal therapy. Chinese Physics B. 2016 Jun 25;25(8):087301.
    17. You J, Zhang G, Li C. Exceptionally high payload of doxorubicin in hollow gold nanospheres for near-infrared light-triggered drug release. ACS nano. 2010 Feb 1;4(2):1033-41.