An Evaluation of Effects of Black Grape and Ginger Extracts on Hematological Alteration and Lipid Peroxidation of Hepatocyte in Irradiated Albino Mice

Document Type : Original Paper

Authors

1 Technical College of Health Medical Laboratory Department Sulaimaniyah Polytechnic University

2 Technical College of Health Anesthesia Department Sulaimaniyah Polytechnic University

3 Medical laboratory departmenttechnical college of health sulaimaniyah polytechnic university

4 Zhianawa Cancer Center (ZCC) Medical Physics Department

5 College of Education Biology Department University of Sulaimaniyah

Abstract

Introduction: Exposure to ionizing radiation can trigger adverse biological effects on healthy tissues, such as causing hematological toxicity and potential injury to different organs. Ionizing radiation has sufficient energy to liberate electrons from atoms leaving them with unpaired electrons; hence ionizing them and producing free radicals. In the current study, the risk of exposing to 6 Gy x-irradiation on alteration of some hematological parameters and liver tissue lipid peroxidation activity in albino mice in the presence and absence of black grape and ginger extracts as antioxidants have been investigated.
Material and Methods: Albino mice were exposed to 6 Gy wholebody xirradiation in the absence and presence of black grape and ginger extracts (10mL/Kg).
Results: The results of the present study showed a significant decrease in mice red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, and mean corpuscular hemoglobin, following the exposure to 6 Gy x-irradiation (P≤0.05), while all the mentioned parameters were relatively shifted toward the normal values in the mice that received black grape and ginger extracts as a treatment prior to the radiation. Accordingly, our results demonstrated a noticeable increase in the malondialdehyde analysis (MDA) level in the 6 Gy group, while a slight depletion in the MDA level was observed in the group of mice that received black grape and ginger extracts, compared to that of the control group. 
Conclusion: the administration of black grape and ginger extracts prior to irradiation may protect the mice from excess hepatocyte lipid peroxidation and alteration of hematological parameters.

Keywords

Main Subjects

References

  1. References

     

    1. Jung J, Kim H, Yoon S, Cho B, Kim Y, Kwak J, et al. Targeting Accuracy of Image-Guided Stereotactic Body Radiation Therapy for Hepatocellular Carcinoma in Real-Life Clinical Practice: In Vivo Assessment Using Hepatic Parenchymal Changes on Gd-EOB-DTPAEnhanced Magnetic Resonance Images. International Journal of Radiation Oncology*Biology*Physics. 2018; 102(4):867-74.
    2. Guha C, Kavanagh BD. Hepatic radiation toxicity: avoidance and amelioration. InSeminars in radiation oncology. 2011;21(4): 256-63.
    3. Lawrence TS, Robertson JM, Anscher MS, Jirtle RL, Ensminger WD, Fajardo LF. Hepatic toxicity resulting from cancer treatment. International Journal of Radiation Oncology* Biology* Physics. 1995;31(5):1237-48.
    4. Kobashigawa S, Kashino G, Suzuki K, Yamashita S, Mori H. Ionizing radiation-induced cell death is partly caused by increase of mitochondrial reactive oxygen species in normal human fibroblast cells. Radiat. Res. 2015; 183: 455–64.
    5. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: impact on human health. Pharmacogn Rev. 2010; 4:118–26.
    6. Smirnova OA. Environmental radiation effects on mammals: a dynamical modeling approach. Springer; 2016.
    7. Akushevich IV, Veremeyeva GA, Dimov GP, Ukraintseva SV, Arbeev KG, Akleyev AV, et al. Modeling hematopoietic system response caused by chronic exposure to ionizing radiation. Radiation and environmental biophysics. 2011;50(2):299-311.
    8. Rösler P, Christiansen H, Kortmann RD, Martini C, Matuschek C, Meyer F, et al. Hepatotoxicity after liver irradiation in children and adolescents. Strahlentherapie und Onkologie. 2015;191(5):413-20.
    9. Kim J, Jung Y. Radiation-induced liver disease: current understanding and future perspectives. Experimental & molecular medicine. 2017;49(7):359.
    10. Ozasa K, Shimizu Y, Suyama A, Kasagi F, Soda M, Grant EJ, et al. Studies of the mortality of atomic bomb survivors, Report 14, 1950–2003: an overview of cancer and noncancer diseases. Radiation research. 2011;177(3):229-43.
    11. Zielinski J, Garner M, Band P, Krewski D, Shilnikova N, Jiang H, et al. Health outcomes of low-dose ionizing radiation exposure among medical workers: a cohort study of the Canadian national dose registry of radiation workers. International journal of occupational medicine and environmental health. 2009;22(2):149-56.
    12. De Freitas R, Augusti P, De Andrade E, Rother F, Rovani B, Quatrin A, et al. Black Grape Juice Protects Spleen from Lipid Oxidation Induced by Gamma Radiation in Rats. Journal of Food Biochemistry. 2012;38(1):119-27.
    13. Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. Rsc Advances. 2015;5(35):27986-8006.
    14. Niki E, Yoshida Y, Saito Y, Noguchi N. Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochemical and biophysical research communications. 2005;338(1):668-76.
    15. Sree KK, Srinivasan V, Toles R, Jobe L, Seed TM. Nutritional approaches to radioprotection: vitamin E. Military medicine. 2002;167(suppl_1):57-9.
    16. Andrade ER, Cruz IB, Andrade VV, Piccoli JC, González-Gallego J, Barrio JP, et al. Evaluation of the potential protective effects of ad libitum black grape juice against liver oxidative damage in whole-body acute X-irradiated rats. Food and chemical toxicology. 2011;49(4):1026-32.
    17. Mashhadi NS, Ghiasvand R, Askari G, Hariri M, Darvishi L, Mofid MR. Anti-oxidative and anti-inflammatory effects of ginger in health and physical activity: review of current evidence. International journal of preventive medicine. 2013;4(Suppl 1):36.
    18. Zhou K, Raffoul JJ. Potential anticancer properties of grape antioxidants. Journal of oncology. 2012;2012.
    19. Grases F, Prieto RM, Fernández-Cabot RA, Costa-Bauzá A, Sánchez AM, Prodanov M. Effect of consuming a grape seed supplement with abundant phenolic compounds on the oxidative status of healthy human volunteers. Nutrition journal. 2015;14(1):94.
    20. Rahmani AH. Active ingredients of ginger as potential candidates in the prevention and treatment of diseases via modulation of biological activities. International journal of physiology, pathophysiology and pharmacology. 2014;6(2):125.
    21. Samarth RM, Samarth M, Matsumoto Y. Medicinally important aromatic plants with radioprotective activity. Future science OA. 2017;3(4):FSO247.
    22. Surh YJ. Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 1999;428(1-2):305-27.
    23. VÁZquez-Armenta FJ, Bernal-Mercado AT, Pacheco-Ordaz R, Gonzalez-Aguilar GA, Ayala-Zavala JF. Winery and grape juice extraction by-products. InPlant Food By-Products: Apple Academic Press. 2018;157-81.
    24. Liu M, Xia X, Chou G, Liu D, Zuberi A, Ye J, et al. Variations in the Contents of Gingerols and Chromatographic Fingerprints of Ginger Root Extracts Prepared by Different Preparation Methods. Journal of AOAC International. 2014;97(1):50-7.
    25. De Freitas RB, Augusti PR, De Andrade ER, Rother FC, Rovani BT, Quatrin A, et al. Black grape juice protects spleen from lipid oxidation induced by gamma radiation in rats. Journal of food biochemistry. 2014 Feb;38(1):119-27.
    26. Xu D, Peng M, Zhang Z, Dong G, Zhang Y, Yu H. Study of damage to red blood cells exposed to different doses of γ-ray irradiation. Blood Transfusion. 2012;10(3):321.
    27. Sancheti G, Goyal PK. Prevention of radiation induced hematological alterations by medicinal plant Rosmarinus officinalis, in mice. African Journal of Traditional, Complementary and Alternative Medicines. 2007;4(2):165-72.
    28. Agarwal M, Purohit RK, Chakrawarti A, Basu A, Bhartiya KM. Protective efficacy of Aloe vera against radiation and cadmium induced haematological changes in the Swiss albino mice. Advanced Biotech. 2011;10(10):44-7.
    29. Sharma R, Purohit RK, Sharma S, Rao R, Purohit RK. Protective role of liv. 52 against radiation and cadmium induced haematological changes in the Swiss albino mice. InProceedings of the international conference on emerging frontiers and challenges in radiation biology: abstracts 2012.
    30. El-Deeb AE, IM AE. The curative effect of some antioxidants on γ-irradiated rats. J. Egypt. Soc. Toxicol. 2006;35:79-89.
    31. Abojassim AA, Jaffat HS, Hassan AB. Effects of gamma radiation on some hematological parameters in female rats. Theoretical & Applied Science. 2015(5):101-9.
    32. Bloom ML, Simon-Stoos KL. The hemoglobin-deficit mouse: analysis of phenotype and hematopoiesis in the transplant model. Blood. 1997;90(5):2062-7.
    33. Abdelhalim MA, Al-Ayed MS, Moussa SA, Al-Shehri AE. The effects of gamma-radiation on red blood cell corpuscles and dimensional properties in rats. Pak. J. Pharm. Sci. 20151;28(5):1819-22.
    34. Daga SS, Jain VK, Goyal PK. Radioresponse to leucocytes in peripheral blood mice against gamma irradiation and their protection by Liv. 52. Probe. 1995;34(3):222.
    35. Gupta U, Agrawal NK. Prevention of radiation induced hematological alterations in mice by Alstonia scholaris extract. International Journal of Pharmaceutical Sciences and Research. 2013 ;4(4):1453.
    36. Mehrotra S, Pecaut MJ, Gridley DS. Effects of minocycline on hematopoietic recovery after whole-body irradiation. in vivo. 2013;27(1):11-28.
    37. El-Desouky W, Hanafi A, Abbas MM. Radioprotective effect of green tea and grape seed extracts mixture on gamma irradiation induced immune suppression in male albino rats. International journal of radiation biology. 2017;93(4):433-9.
    38. Singha I, Das SK. Grape extract protects against ionizing radiation-induced membrane alterations in Red Blood Cells. 2016.
    39. Jagetia GC. Radioprotective potential of plants and herbs against the effects of ionizing radiation. Journal of clinical biochemistry and nutrition. 2007;40(2):74-81.
    40. Painuli S, Kumar N. Prospects in the development of natural radioprotective therapeutics with anti-cancer properties from the plants of Uttarakhand region of India. Journal of Ayurveda and integrative medicine. 2016;7(1):62-8.
    41. Kiang JG, Fukumoto R, Gorbunov NV. Lipid peroxidation after ionizing irradiation leads to apoptosis and autophagy. InLipid peroxidation. 2012.
    42. Nakajima T, Ninomiya Y, Nenoi M. Radiation-Induced Reactions in the Liver—Modulation of Radiation Effects by Lifestyle-Related Factors—. International journal of molecular sciences. 2018;19(12):3855.
Iranian Journal of Medical Physics
Volume 17, Issue 4 - Serial Number 4
July and August 2020
Pages 247-252

Files

History

  • Receive Date: 12 May 2019
  • Revise Date: 16 September 2019
  • Accept Date: 16 September 2019

Share

How to cite

Statistics