Expression of DDB2, XPC, and GADD45 Genes after Whole Body Gamma Irradiation

Document Type : Original Paper

Authors

1 Department of Radiology, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran & Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran

2 Department of Radiology, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

3 Department of Radiology, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences

4 Department of Radiology, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran & 2Ionizing and Non-Ionizing Radiation Protection Research Center (INIRPRC), Shiraz University of Medical Sciences, Shiraz, Iran

5 Department of Laboratory Medicine, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

Abstract

Purpose

The stimulation of DNA repair mechanisms is an immediate response to IR-induced damage. Monitoring the expression of DNA-repair-related genes would be a beneficial method to identify bio-dosimeter of radiation exposure, particularly for a challenging low dose radiation. In this study, we aimed to evaluate the effect of different low-doses of gamma radiation on the expression of DDB2, XPC, and GADD45A involved in DNA-damage repair mechanisms.

Method and Materials

Forty-eight male rats were divided into a control group and five exposure groups. The latter groups exposed to various doses of γ-rays (Co-60) ranged from 20 mGy to 1000 mGy. 24 h after radiation, isolated lymphocytes from collected blood samples were used for evaluating gene expression levels by real-time qRT-PCR. Data were expressed as means ± SD and were statistically evaluated using the one‑way ANOVA Tukey‑Krammer test. P value<0.05 was considered as significant value.

Results

DDB2, GADD45A, and XPC expression remained unchanged at a dose of 20 mGy, and at doses above 20 mGy, they changed significantly. XPC and GADD45A altered significantly at 50 mGy while DDB2 changed significantly after exposure to 100, 500, and 1000 mGy.

Conclusion

Low doses of gamma radiation (less than 1 Gy) can significantly affect DDB2, XPC, and GADD45A expression, three central genes in the DNA damage repair process. The extent of the gene expression changes at higher doses of 100, 500, and 1000 mGy seems more severe than that of their lower counterparts (50 mGy).

Keywords

Main Subjects



Articles in Press, Accepted Manuscript
Available Online from 28 September 2021
  • Receive Date: 18 July 2021
  • Revise Date: 21 September 2021
  • Accept Date: 28 September 2021