Evaluation of Contrast to Noise Ratio (CNR) of Targeted and Non-Targeted Gold nanoparticles in nasopharyngeal cancer cells in CT images

Document Type : Original Paper

Authors

1 Department of Radiology Technology, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Medical Physics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Introduction: In this study, we aimed to identify the benefits in image contrast enhancement of Folic acid-Cysteamine conjugated gold nanoparticles (FA-Cys-AuNPs) using measuring Contrast to Noise (CNR) compared to gold nanoparticles (AuNPs) and iodinated contrast agent. The CNR was assessed in different tube voltages, concentrations, and incubation times in nasopharyngeal KB cancer cells.

Materials and Methods: FA-Cys-AuNPs and Omnipaque suspension were scanned at different concentration ranges (500-2000 µg/ml) and energy ranges (80- 140 kVp) with CT imaging modality. FA-Cys-AuNPs and AuNPs were incubated in nasopharyngeal cancer cells at different incubation times (6, 12, and 24 h) and concentration ranges (200-500 µM). Finally, the contrast enhancement was assessed using CNR value at different tube voltages.

Results: Results showed that the formed FA-Cys-AuNPs with an Au core size of 15 nm in all concentrations and tube potentials from 80 to 140 kVp display greater CNR than Omnipaque. The CNR value was increased by increasing concentration and energy. At 140 kVp and 2000 µg/ml, the CNR value of FA-Cys-AuNPs was 2.25 times greater than Omnipaque. At 140 kVp, 500 µM and 24 h incubation, the CNR value of targeted cells were approximately 1.5 times higher than non-targeted cells. At 140 kVp, and 500 µM, the CNR value of targeted cells with 24 h incubation time was 2.66 times greater than the targeted cells incubated with 6 h.

Conclusion: These findings suggested that the designed FA-Cys-AuNPs could be a good candidate contrast agent for molecular CT imaging.

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Articles in Press, Accepted Manuscript
Available Online from 22 January 2022
  • Receive Date: 29 November 2021
  • Revise Date: 05 March 2022
  • Accept Date: 22 January 2022
  • First Publish Date: 22 January 2022