Iranian Journal of Medical Physics

Iranian Journal of Medical Physics

Evaluation of Contrast to Noise Ratio of Targeted and Non-Targeted Gold nanoparticles in nasopharyngeal cancer cells in CT images

Document Type : Original Paper

Authors
Sara Khademi 1
Hosein Azimian 2
Hossein Ghadiri 3
1 Department of Radiology Technology, School of Paramedical and Rehabilitation Sciences, Mashhad University of Medical Sciences, Mashhad, Iran
2 Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
3 Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Sciences, Tehran, Iran
10.22038/ijmp.2022.61916.2046
Abstract
Introduction: In this study, we aimed to identify the benefits in image contrast enhancement of Folic acid-Cysteamine conjugated gold nanoparticles (FA-Cys-AuNPs) by comparing Contrast to Noise ratio (CNR) of gold nanoparticles (AuNPs) to iodinated compound CT images. The CNR was assessed in different tube voltages, concentrations, and incubation times in nasopharyngeal KB cancer cells.
Material and Methods: FA-Cys-AuNPs and Omnipaque suspension were scanned at different concentration ranges (500-2000 µg/ml) and energy ranges (80- 140 kVp) with CT imaging modality. FA-Cys-AuNPs and AuNPs were incubated in nasopharyngeal cancer cells at different incubation times (6, 12, and 24 h) and concentration ranges (200-500 µM). Finally, the contrast enhancement was assessed using CNR value at different tube voltages.
Results: Results showed that the formed FA-Cys-AuNPs with an Au core size of 15 nm in all concentrations and tube potentials from 80 to 140 kVp display greater CNR than Omnipaque. The CNR value was increased by increasing concentration and energy. At 140 kVp and 2000 µg/ml, the CNR value of FA-Cys-AuNPs was 2.25 times greater than Omnipaque. At 140 kVp, 500 µM and 24 h incubation, the CNR values of targeted cells were approximately 1.5 times higher than non-targeted cells. At 140 kVp, and 500 µM, the CNR value of targeted cells with 24 h incubation time was 2.66 times greater than the targeted cells incubated with 6 h.
Conclusion: These findings suggested that the designed FA-Cys-AuNPs could be a good candidate contrast agent for molecular CT imaging.
Keywords
Folic Acid
Molecular Imaging
CT
Gold Nanoparticle
Cancer
Subjects

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    1. Lalumera, E., S. Fanti, and G. Boniolo, Reliability of molecular imaging diagnostics. Synthese, 2021 Oct; 198(23): p. 5701-5717.
    2. Keshtkar, M., D. Shahbazi-Gahrouei, and A. Mahmoudabadi, Synthesis and application of Fe3O4@ Au composite nanoparticles as magnetic resonance/computed tomography dual-modality contrast agent. JMSS, 2020Jul; 10(3): p. 201.
    3. Shakeri-Zadeh A, Rezaeyan A, Sarikhani A, Ghaffari H, Samadian H, Khademi S, Ghaznavi H, Bulte JW. Folate receptor-targeted nanoprobes for molecular imaging of cancer: Friend or foe? Nano Today. 2021 Aug 1;39:101173
    4. Khademi S, Shakeri‐Zadeh A, Solgi R, Azimian H, Ghadiri H. Observation of targeted gold nanoparticles in nasopharyngeal tumour nude mice model through dual‐energy computed tomography. IET Nanobiotechnology, 2021 Sep;15(7):594-601..
    5. Ghaznavi H, Hosseini-Nami S, Kamrava SK, Irajirad R, Maleki S, Shakeri-Zadeh A, Montazerabadi A. Folic acid conjugated PEG coated gold–iron oxide core–shell nanocomplex as a potential agent for targeted photothermal therapy of cancer. Artificial cells, nanomedicine, and biotechnology. 2018 Nov 17;46(8): p.1594-604.
    6. Khademi S, Sarkar S, Shakeri-Zadeh A, Attaran N, Kharrazi S, Ay MR, Ghadiri H. Folic acid-cysteamine modified gold nanoparticle as a nanoprobe for targeted computed tomography imaging of cancer cells. Materials Science and Engineering: C. 2018 Aug 1;89:182-93.
    7. Beik, J., et al., A nanotechnology-based strategy to increase the efficiency of cancer diagnosis and therapy: folate-conjugated gold nanoparticles. Curr. Med. Chem, 2017. 24(39): p. 4399-4416. Beik J, Khademi S, Attaran N, Sarkar S, Shakeri-Zadeh A, Ghaznavi H, Ghadiri H. A nanotechnology-based strategy to increase the efficiency of cancer diagnosis and therapy: folate-conjugated gold nanoparticles. Current medicinal chemistry. 2017 Dec 1;24(39):4399-416.
    8. Beik, J., et al., The benefits of folic acid-modified gold nanoparticles in CT-based molecular imaging: radiation dose reduction and image contrast enhancement. Artif Cells Nanomed Biotechnol, 2018. 46(8): p. 1993-2001. Beik J, Jafariyan M, Montazerabadi A, Ghadimi-Daresajini A, Tarighi P, Mahmoudabadi A, Ghaznavi H, Shakeri-Zadeh A. The benefits of folic acid-modified gold nanoparticles in CT-based molecular imaging: radiation dose reduction and image contrast enhancement. Artificial cells, nanomedicine, and biotechnology. 2018 Nov 17;46(8):1993-2001.
    9. Fan Y, Tu W, Shen M, Chen X, Ning Y, Li J, Chen T, Wang H, Yin F, Liu Y, Shi X. Targeted tumor hypoxia dual‐mode CT/MR imaging and enhanced radiation therapy using dendrimer‐based nanosensitizers. Advanced Functional Materials. 2020 Mar;30(13):1909285.
    10. Montazerabadi, A., et al., Folate-modified and curcumin-loaded dendritic magnetite nanocarriers for the targeted thermo-chemotherapy of cancer cells. Artif Cells Nanomed Biotechnol, 2019. 47(1): p. 330-340. Montazerabadi A, Beik J, Irajirad R, Attaran N, Khaledi S, Ghaznavi H, Shakeri-Zadeh A. Folate-modified and curcumin-loaded dendritic magnetite nanocarriers for the targeted thermo-chemotherapy of cancer cells. Artificial cells, nanomedicine, and biotechnology. 2019 Dec 4;47(1):330-40.
    11. Zeinizade, E., et al., Selective apoptosis induction in cancer cells using folate-conjugated gold nanoparticles and controlling the laser irradiation conditions. Artif Cells Nanomed Biotechnol, 2018. 46(sup1): p. 1026-1038. Zeinizade E, Tabei M, Shakeri-Zadeh A, Ghaznavi H, Attaran N, Komeili A, Ghalandari B, Maleki S, Kamrava SK. Selective apoptosis induction in cancer cells using folate-conjugated gold nanoparticles and controlling the laser irradiation conditions. Artificial cells, nanomedicine, and biotechnology. 2018 Oct 31;46(sup1):1026-38.
    12. Movahedi, M.M., et al., Investigating the photo-thermo-radiosensitization effects of folate-conjugated gold nanorods on KB nasopharyngeal carcinoma cells. Photodiagnosis and photodynamic therapy, 2018. 24: p. 324-331. Movahedi MM, Mehdizadeh A, Koosha F, Eslahi N, Mahabadi VP, Ghaznavi H, Shakeri-Zadeh A. Investigating the photo-thermo-radiosensitization effects of folate-conjugated gold nanorods on KB nasopharyngeal carcinoma cells. Photodiagnosis and Photodynamic Therapy. 2018 Dec 1;24:324-31.
    13. Khademi, S., et al., Targeted gold nanoparticles enable molecular CT imaging of head and neck cancer: an in vivo study. The international journal of biochemistry & cell biology, 2019. 114: p. 105554. Khademi S, Sarkar S, Shakeri-Zadeh A, Attaran N, Kharrazi S, Ay MR, Azimian H, Ghadiri H. Targeted gold nanoparticles enable molecular CT imaging of head and neck cancer: an in vivo study. The international journal of biochemistry & cell biology. 2019 Sep 1;114:105554.
    14. Harun, A.Z., et al., Evaluation of Contrast-Noise Ratio (CNR) in contrast enhanced CT images using different sizes of gold nanoparticles. Mater. Today: Proc., 2019. 16: p. 1757-1765. Harun AZ, Ab Rashid R, Ab Razak K, Geso M, Rahman WN. Evaluation of contrast-noise ratio (CNR) in contrast enhanced CT images using different sizes of gold nanoparticles. Materials Today: Proceedings. 2019 Jan 1;16:1757-65.
    15. Khademi, S., et al., Evaluation of size, morphology, concentration, and surface effect of gold nanoparticles on X-ray attenuation in computed tomography. Physica Medica, 2018. 45: p. 127-133. Khademi S, Sarkar S, Kharrazi S, Amini SM, Shakeri-Zadeh A, Ay MR, Ghadiri H. Evaluation of size, morphology, concentration, and surface effect of gold nanoparticles on X-ray attenuation in computed tomography. Physica Medica. 2018 Jan 1;45:127-33.
    16. Jackson, P.A., et al., Potential dependent superiority of gold nanoparticles in comparison to iodinated contrast agents. Eur. J. Radiol., 2010. 75(1): p. 104-109. Jackson PA, Rahman WN, Wong CJ, Ackerly T, Geso M. Potential dependent superiority of gold nanoparticles in comparison to iodinated contrast agents. European journal of radiology. 2010 Jul 1;75(1):104-9.
    17. Kim, J., et al., Use of nanoparticle contrast agents for cell tracking with computed tomography. Bioconjugate chemistry, 2017. 28(6): p. 1581-1597 Kim J, Chhour P, Hsu J, Litt HI, Ferrari VA, Popovtzer R, Cormode DP. Use of nanoparticle contrast agents for cell tracking with computed tomography. Bioconjugate chemistry. 2017 Jun 21;28(6):1581-97..
    18. Kim, D., et al., Antibiofouling polymer-coated gold nanoparticles as a contrast agent for in vivo X-ray computed tomography imaging. JACS, 2007. 129(24): p. 7661-7665. Kim D, Park S, Lee JH, Jeong YY, Jon S. Antibiofouling polymer-coated gold nanoparticles as a contrast agent for in vivo X-ray computed tomography imaging. Journal of the American Chemical Society. 2007 Jun 20;129(24):7661-5.
    19. Hu, Y., et al., Multifunctional Fe 3 O 4@ Au core/shell nanostars: a unique platform for multimode imaging and photothermal therapy of tumors. Sci. Rep., 2016. 6(1): p. 1-12. Hu Y, Wang R, Wang S, Ding L, Li J, Luo Y, Wang X, Shen M, Shi X. Multifunctional Fe3O4@ Au core/shell nanostars: a unique platform for multimode imaging and photothermal therapy of tumors. Scientific reports. 2016 Jun 21;6(1):1-2.

     

     

     

     

     

Iranian Journal of Medical Physics
Volume 20, Issue 1
January and February 2023
Pages 42-47

  • Receive Date 29 November 2021
  • Revise Date 05 March 2022
  • Accept Date 22 January 2022