Evaluation of Sinensetin Derived From Flavonoids-Rich Orthosiphon Stamineus as a New Radiosensitizer in MDA-MB-231 and T47D as Breast Cancer Cell Lines

Document Type : Original Paper

Authors

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran

3 Department of Biology, Islamshahr Branch, Islamic Azad University, Tehran, Iran

4 Department of Radiotherapy, Iran University of Medical Sciences (IUMS), Tehran, Iran

5 Department of Photo Healing and Regeneration, Medical Laser Research Center, Yara Institute, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran

6 Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, IRAN

7 Department of Biophysics, Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.

Abstract

Introduction: Breast cancer is one of the most prevalent diseases around the world. Breast cancer patients treated with radiation may face Side effects as well as cancer recurrence. Some polyphenols exhibit antioxidant effects. Citrus and Orthosiphon Stamineus have source of methylated flavone called Sinensetin (SIN). In this research, we designation of Sinensetin as increasing radiation sensitivity.
Material and Methods: The cytotoxic effect of Sinensetin was examined in MDA-MB-231 and T47D by MTT assay. As well as, the clonogenic ability of cells were Assessmented in the presence of Sinensetin and combination with radiation. To quantify expression alterations of apoptosis related genes, utilized Real-Time PCR method.
Results: In a dose and time-dependent manner, Sinensetin decreased the viability of MDA-MB-231 and T47D. The survival fraction was decreased in cells treated with Sinensetin (SIN) prior to X-irradiation compared to cells treated with X-ray only. More ever, expression level of, Bcl-2, STAT3, and increased P53 via treated cells with Sinensetin (SIN) and X-ray.
Conclusion: Due to the results, Sinensetin (SIN) can be mentioned as a novel radiosensitizer and its effects may considered increasing apoptosis following DNA damage induced by irradiation.

Keywords

Main Subjects

References

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Iranian Journal of Medical Physics
Volume 20, Issue 2
March and April 2023
Pages 114-119

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History

  • Receive Date: 14 March 2022
  • Revise Date: 05 July 2022
  • Accept Date: 10 July 2022

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