Evaluation and Comparison of Iodine-Based and Biosynthesized Gold Nanoparticles as X-Ray Contrast Agent and Their Effects on Renal Functions in Male Wistar Rats

Document Type : Original Paper

Authors

1 Department of Pure and Applied Physics, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso

2 Department of Physiology, Faculty of Basic Medical Sciences, Ladoke Akintola University of Technology, Ogbomoso

3 Department of Pure and Applied Physics, Faculty of Pure and Applied Physics, Ladoke Akintola University of Technology, Ogbomoso

4 Department of Science Laboratory Technology, Faculty of Pure and Applied Sciences, Ladoke Akintola University of Technology, Ogbomoso

10.22038/ijmp.2024.74142.2316

Abstract

Introduction: Gold nanoparticles (AuNPs) have gained significant attention as a promising contrast agent to overcome the challenges of iodine-based contrast agents (ICAs), such as poor contrast, and renal toxicity. However, studies have associated toxicity with AuNPs synthesized through chemical methods, thereby raising concerns regarding the safety of such AuNPs. This study, hereby, compares the performance of iodine-based and biosynthesized AuNPs from the extract of Psidium guajava and Corchorus olitorius as CA on radiographs and renal functions of rats.
Material and Methods: 156 rats were grouped into baseline (A), experimental control (B), ICA (C), and two AuNPs groups (D and E). The ICA (Urografin 76%), AuNPs, 8.23±1.03 nm (from P. guajava), and 6.57±1.62 nm (from C. olitorius) were administered to the rats in groups C, D, and E respectively. The rats, excluding group A, were radiographed, and samples of blood and kidney tissue were collected on days 1 and 30 for the assessment of renal functions.
Results: The rats injected with the AuNPs showed radiographic contrast with anatomical features better than those without CA and those injected with the ICA. The biochemicals showed a significant decrease in renal functions for the rats injected with the ICA while those injected with the AuNPs protected against nephropathy when compared with those without CA and those injected with ICA.
Conclusion: It was revealed that biosynthesized AuNPs can be a suitable replacement for the ICA because of the high atomic number and attenuation properties of gold, the increased surface area of AuNPs, and biocompatibility.

Keywords

Main Subjects

References

  1. Bae KT. Intravenous contrast medium administration and scan timing at CT: considerations and approaches. Radiology. 2010 Jul;256(1):32-61.
  2. Ayanlola PS, Isola GA, Saka WA, Akinloye MK, Sanusi YK, Adedokun O, et al. Cardioprotective role of biosynthesized gold nanoparticles compared to an iodinated-based x-ray contrast agent in Male Wistar Rats. Nanomedicine Research Journal. 2023 Mar 1;8(1):24-36.
  3. Bushberg JT, Boone JM. The essential physics of medical imaging. Lippincott Williams & Wilkins; 2011 Dec 20.
  4. Suetens P. Fundamentals of medical imaging. Cambridge university press; 2017 May 11.
  5. Nash K, Hafeez A, Hou S. Hospital-acquired renal insufficiency. American Journal of Kidney Diseases. 2002 May 1;39(5):930-6.
  6. Stacul F, Van der Molen AJ, Reimer P, Webb JA, Thomsen HS, Morcos SK, et al. Contrast induced nephropathy: updated ESUR contrast media safety committee guidelines. European radiology. 2011 Dec;21:2527-41.
  7. Faucon AL, Bobrie G, Clément O. Nephrotoxicity of iodinated contrast media: From pathophysiology to prevention strategies. European Journal of Radiology. 2019 Jul 1;116:231-41.
  8. Ludwig U, Keller F. Prophylaxis of contrast‐induced nephrotoxicity. BioMed research international. 2014;2014(1):308316.
  9. Bhatt S, Rajpal N, Rathi V, Avasthi R. Contrast induced nephropathy with intravenous iodinated contrast media in routine diagnostic imaging: an initial experience in a tertiary care hospital. Radiology research and practice. 2016;2016(1):8792984.
  10. Shams E, Mayrovitz HN. Contrast-induced nephropathy: a review of mechanisms and risks. Cureus. 2021 May;13(5).
  11. Dröge W. Free radicals in the physiological control of cell function. Physiological reviews. 2002.
  12. Genestra M. Oxyl radicals, redox-sensitive signalling cascades and antioxidants. Cellular signalling. 2007 Sep 1;19(9):1807-19.
  13. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. The international journal of biochemistry & cell biology. 2007 Jan 1;39(1):44-84.
  14. Rajendran P, Nandakumar N, Rengarajan T, Palaniswami R, Gnanadhas EN, Lakshminarasaiah U, et al. Antioxidants and human diseases. Clinica chimica acta. 2014 Sep 25;436:332-47.
  15. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, et al. Oxidative stress: harms and benefits for human health. Oxidative medicine and cellular longevity. 2017;2017(1):8416763.
  16. Halliwell B. Antioxidants in human health and disease. Annual review of nutrition. 1996 Jul;16(1):33-50.
  17. Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. Japi. 2004 Oct 25;52(794804):4.
  18. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy reviews. 2010 Jul;4(8):118.
  19. Finkel T. Signal transduction by reactive oxygen species. Journal of Cell Biology. 2011 Jul 11;194(1):7-15.
  20. Ray PD, Huang BW, Tsuji Y. Reactive oxygen species (ROS) homeostasis and redox regulation in cellular signaling. Cellular signalling. 2012 May 1;24(5):981-90.
  21. Phaniendra A, Jestadi DB, Periyasamy L. Free radicals: properties, sources, targets, and their implication in various diseases. Indian journal of clinical biochemistry. 2015 Jan;30:11-26.
  22. Katerji M, Filippova M, Duerksen-Hughes P. Approaches and methods to measure oxidative stress in clinical samples: Research applications in the cancer field. Oxidative medicine and cellular longevity. 2019;2019(1):1279250.
  23. Dasgupta A, Wahed A. Clinical chemistry, immunology and laboratory quality control: a comprehensive review for board preparation, certification and clinical practice.
  24. Liss P, Nygren A, Erikson U, Ulfendahl HR. Injection of low and iso-osmolar contrast medium decreases oxygen tension in the renal medulla. Kidney international. 1998 Mar 1;53(3):698-702.
  25. Rosenberger C, Rosen S, Heyman SN. Renal parenchymal oxygenation and hypoxia adaptation in acute kidney injury. Clinical and experimental pharmacology & physiology. 2006 Oct 1;33(10):980-8.
  26. Pisani A, Riccio E, Andreucci M, Faga T, Ashour M, Di Nuzzi A, et al. Role of reactive oxygen species in pathogenesis of radiocontrast‐induced nephropathy. BioMed research international. 2013;2013(1):868321.
  27. Hainfeld JF, Slatkin DN, Focella TM, Smilowitz HM. Gold nanoparticles: a new X-ray contrast agent. The British journal of radiology. 2006 Mar 1;79(939):248-53.
  28. Cormode DP, Skajaa T, Fayad ZA, Mulder WJ. Nanotechnology in medical imaging: probe design and applications. Arteriosclerosis, thrombosis, and vascular biology. 2009 Jul 1;29(7):992-1000.
  29. De La Vega JC, Häfeli UO. Utilization of nanoparticles as X‐ray contrast agents for diagnostic imaging applications. Contrast media & molecular imaging. 2015 Mar;10(2):81-95.
  30. Al-Neami AQ, Al-Karam LQ, Humadi MD, Alwan MH. Applications and advantages of gold nanoparticles as X-ray contrast agent. J Biomed Eng Med Devic. 2017;2(2):1-3.
  31. Gharatape A, Salehi R. Recent progress in theranostic applications of hybrid gold nanoparticles. European journal of medicinal chemistry. 2017 Sep 29;138:221-33.
  32. Guo J, Rahme K, He Y, Li LL, Holmes JD, O’Driscoll CM. Gold nanoparticles enlighten the future of cancer theranostics. International journal of nanomedicine. 2017 Aug 22:6131-52.
  33. Seltzer SE, Blau M, Herman LW, Hooshmand RL, Herman LA, Adams DF, et al. Contrast material-carrying liposomes: biodistribution, clearance, and imaging characteristics. Radiology. 1995 Mar;194(3):775-81.
  34. Isola GA, Akinloye MK, Sanusi YK, Ayanlola PS, Alamu GA. Optimizing X-Ray Imaging Using Plant Mediated Gold Nanoparticles as Contrast Agent: A Review. International Journal of Research and Scientific Innovation. 2021;8(8):169-75.
  35. Jadoun S, Arif R, Jangid NK, Meena RK. Green synthesis of nanoparticles using plant extracts: A review. Environmental Chemistry Letters. 2021 Feb;19(1):355-74.
  36. Sultana S, Djaker N, Boca-Farcau S, Salerno M, Charnaux N, Astilean S, et al. Comparative toxicity evaluation of flower-shaped and spherical gold nanoparticles on human endothelial cells. Nanotechnology. 2015 Jan 9;26(5):055101.
  37. Bhamidipati M, Fabris L. Multiparametric assessment of gold nanoparticle cytotoxicity in cancerous and healthy cells: the role of size, shape, and surface chemistry. Bioconjugate chemistry. 2017 Feb 15;28(2):449-60.
  38. Pujalté I, Passagne I, Brouillaud B, Tréguer M, Durand E, Ohayon-Courtès C, et al. Cytotoxicity and oxidative stress induced by different metallic nanoparticles on human kidney cells. Particle and fibre toxicology. 2011 Dec;8:1-6.
  39. Kermanizadeh A, Vranic S, Boland S, Moreau K, Baeza-Squiban A, Gaiser BK, et al. An in vitro assessment of panel of engineered nanomaterials using a human renal cell line: cytotoxicity, pro-inflammatory response, oxidative stress and genotoxicity. BMC nephrology. 2013 Dec;14:1-2.
  40. Jha AK, Prasad K, Prasad K, Kulkarni AR. Plant system: nature's nanofactory. Colloids and Surfaces B: Biointerfaces. 2009 Oct 15;73(2):219-23.
  41. Thakkar KN, Mhatre SS, Parikh RY. Biological synthesis of metallic nanoparticles. Nanomedicine: nanotechnology, biology and medicine. 2010 Apr 1;6(2):257-62.
  42. Yasmin A, Ramesh K, Rajeshkumar S. Optimization and stabilization of gold nanoparticles by using herbal plant extract with microwave heating. Nano convergence. 2014 Apr 11;1(1):12.
  43. Kumar M, Tomar M, Amarowicz R, Saurabh V, Nair MS, Maheshwari C, et al. Guava (Psidium guajava L.) leaves: Nutritional composition, phytochemical profile, and health-promoting bioactivities. Foods. 2021 Apr 1;10(4):752.
  44. Ismail EH, Saqer AM, Assirey E, Naqvi A, Okasha RM. Successful green synthesis of gold nanoparticles using a Corchorus olitorius extract and their antiproliferative effect in cancer cells. International journal of molecular sciences. 2018 Sep 3;19(9):2612.
  45. Yokoyama S, Hiramoto K, Fujikawa T, Kondo H, Konishi N, Sudo S, et al. Topical application of Corchorus olitorius leaf extract ameliorates atopic dermatitis in NC/Nga mice. Dermatol Aspects. 2014;2(3):10-7243.
  46. National Research Council. Guide for the Care and Use of Laboratory Animals Eighth Edition. Washington, DC: The National Academies Press. 2011
  47. Turner PV, Brabb T, Pekow C, Vasbinder MA. Administration of substances to laboratory animals: routes of administration and factors to consider. Journal of the American Association for Laboratory Animal Science. 2011 Sep 15;50(5):600-13.
  48. Abdelhalim MA, Moussa SA. The gold nanoparticle size and exposure duration effect on the liver and kidney function of rats: In vivo. Saudi journal of biological sciences. 2013 Apr 1;20(2):177-81.
  49. Pandy V. A simple method for animal dose calculation in preclinical research. EC Pharmacol Toxicol. 2020;8:1-2.
  50. Halliwell B, Gutteridge JC. Lipid peroxidation, oxygen radicals, cell damage, and antioxidant therapy. The Lancet. 1984 Jun 23;323(8391):1396-7.
  51. Chaudière J, Ferrari-Iliou R. Intracellular antioxidants: from chemical to biochemical mechanisms. Food and chemical toxicology. 1999 Sep 1;37(9-10):949-62.
  52. Forsyth P, Fleming L, Moir L, Mcclusky L, Watson N, Meiklejohn J, et al. Improving medication adherence in patients with chronic heart failure: a feasibility study.
  53. Fedorova S, Miyamoto R, Harada T, Isogai S, Hashimoto H, Ozato K, et al. Renal glomerulogenesis in medaka fish, Oryzias latipes. Developmental Dynamics: An Official Publication of the American Association of Anatomists. 2008 Sep;237(9):2342-52.
  54. Kaid F, Alabsi AM, Alafifi N, Ali-Saeed R, Ameen Al-koshab M, Ramanathan A, et al. Histological, biochemical, and hematological effects of goniothalamin on selective internal organs of male sprague-dawley rats. Journal of Toxicology. 2019;2019(1):6493286.
  55. Zhang Z, Ross RD, Roeder RK. Preparation of functionalized gold nanoparticles as a targeted X-ray contrast agent for damaged bone tissue. Nanoscale. 2010;2(4):582-6.
  56. Bashandy MM, Ahmed AR, El-Gaffary M, Abd El-Rahman SS. Gold nanoparticle: synthesis, characterization, clinico-pathological, pathological, and bio-distribution studies in rabbits. International Journal of Pharmacological and Pharmaceutical Sciences. 2015 Nov 1;9(11):1168-74.
  57. He Z, Li C, Zhang X, Zhong R, Wang H, Liu J, et al. The effects of gold nanoparticles on the human blood functions. Artificial cells, nanomedicine, and biotechnology. 2018 Nov 5;46(sup2):720-6.
  58. Lee JH, Gulumian M, Faustman EM, Workman T, Jeon K, Yu IJ. Blood biochemical and hematological study after subacute intravenous injection of gold and silver nanoparticles and coadministered gold and silver nanoparticles of similar sizes. BioMed research international. 2018;2018(1):8460910.
  59. Isoda K, Tanaka A, Fuzimori C, Echigoya M, Taira Y, Taira I, et al. Toxicity of gold nanoparticles in mice due to nanoparticle/drug interaction induces acute kidney damage. Nanoscale research letters. 2020;15:1-8.
  60. Aljohani FS, Hamed MT, Bakr BA, Shahin YH, Abu-Serie MM, Awaad AK, et al. In vivo bio-distribution and acute toxicity evaluation of greenly synthesized ultra-small gold nanoparticles with different biological activities. Scientific reports. 2022 Apr 15;12(1):6269.

 

 

 

 

 

Iranian Journal of Medical Physics
Volume 21, Issue 5 - Serial Number 5
September and October 2024
Pages 295-305

Files

History

  • Receive Date: 03 August 2023
  • Revise Date: 14 May 2024
  • Accept Date: 19 May 2024

Share

How to cite

Statistics