Iranian Journal of Medical Physics

Iranian Journal of Medical Physics

Anti-Apoptosis Effects of Green Tea in Diagnostic and Therapeutic Doses of Ionizing Radiation

Document Type : Original Paper

Authors
Najmeh Anbiaee 1
Mohammad Taghi Bahreyni Toossi 2
Hosein Azimian 3
Zoha Sahebnasagh 1
Sepideh Abdollahi Dehkordi 4
Majid Kianmehr 5
samaneh soudmand salarabadi 6
Maryam Najafi Amiri 3
1 Department of Oral and Maxillofacial Radiology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
2 Medical Physics Department, Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
3 Department of Medical Physics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4 Department of Medical Physics, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
5 Esfarayen Faculty of Medical Sciences, Esfarayen, Iran.
6 Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
10.22038/ijmp.2024.74945.2330
Abstract
Introduction: Patients who receive ionizing radiation for diagnosis or treatment may suffer side effects in normal tissues. Radioprotective agents have the potential to decrease DNA damage and free radicals produced by ionizing radiation. Green tea is a natural product from the Camellia sinensis plant and have been shown to have antioxidant and radioprotective properties. The aim of the present study is assessing the protective effect of green tea on lymphocytes affected by radiation injury.
Material and Methods: Blood samples was obtained from four adults healthy human and lymphocytes were extracted. Afterward, lymphocytes were exposed to X-radiation by OPG, CBCT, CT and radiotherapy instruments. The protective effects of the green tea polyphenol on Bcl-2 and Bax genes expression levels were evaluated in cultured lymphocytes obtained from treated cells (24 h and 1 h before and 1 h after exposure to ionizing radiation) and non-treated cells (Receiving X-radiation and non-receiving green tea).
Results: The results of this investigation demonstrated Bax/Bcl-2 ratio, as an index of the radiation sensitivity, was significantly decreased in groups which had been received dose of 2 Gy in treated cells groups of 1 h before and 1 h after X-radiation (p<0.05).
Conclusion: The results indicated different effects of green tea on low and high doses of X-radiation. Most protective effects of green tea as an apoptosis inhibitor were observed in high doses of ionizing radiation. Consequently, green tea may be useful as an adjunct to radiotherapy and medical imaging to diminish apoptosis and side effects in normal tissues.
Keywords
Apoptosis
Gene Expression
Radiotherapy
Diagnostic Techniques
Polyphenol
Green Tea
Subjects
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Iranian Journal of Medical Physics
Volume 21, Issue 4 - Serial Number 4
July and August 2024
Pages 222-227

  • Receive Date 10 October 2023
  • Revise Date 28 September 2024
  • Accept Date 04 March 2024