Investigation of Biophysical Molecular Properties by Temperature Influence on the Silver Nanoparticles Synthesized Via Green Methods Using Beta Vulgaris: Applications of Antibacterial Agents

Document Type : Original Paper

Authors

Department of Medical physics, College of Science, AL-Karkh University of Science. Baghdad, Iraq

10.22038/ijmp.2025.86104.2513

Abstract

Introduction: Beta vulgaris has traditionally been utilized for the treatment of anemia, cancer prevention, fever management, anticoagulation, sclerosis, and bodily detoxification. Beets have been utilized for these purposes by numerous ancient tribes throughout antiquity.
Material and Methods: The major purpose of this research was to investigate the impact of betalains derived from beetroot (Beta vulgaris) on the biosynthesis of silver nanoparticles (AgNPs).
Results: The in vitro antibacterial effectiveness of Beta vulgaris L. pomace extract was evaluated at a dose of 100 mg/ml against clinical isolates and reference strains of Escherichia coli and Klebsiella pneumoniae. Well diffusion (50, 100 µl) and disc diffusion (15 µl) were other methods utilized. Fourier transform infrared spectroscopy, scanning tunneling microscopy (STM), and scanning electron microscopy (SEM) were employed to analyze the physical properties of the synthesized silver nanoparticles. Antimicrobial characteristics, silver nanoparticles, agar well-diffusion method.
Conclusion: This distinctive green synthesis technique for generating silver nanoparticles obviates the necessity for detrimental solvents and byproducts, rendering it an optimal instrument for promoting green technology in nanotechnology.

Keywords

Main Subjects


  1. Nandhini SN, Sisubalan N, Vijayan A, Karthikeyan C, Gnanaraj M, Gideon DAM, et al. Recent advances in green synthesized nanoparticles for bactericidal and wound healing applications. Heliyon. 2023;9(2):e13128.
  2. Mohammed RA, Mutlak FA, Saleh GM. Structural and optical properties of green spinach extract leaf (Spinacia oleracea) prepared with silver nanoparticles as antibacterial by effect of pulsed laser. Journal of Optics. 2021;51(2):1-9.
  3. Alattar AM. The influence of pulsed laser on the structural and optical properties of green tea extract leaf produced with silver nanoparticles as antimicrobial. Journal of Molecular Liquids. 2024;398:124287.
  4. Alattar AM, Al-tememee NA. Preparation and analysis of silver nanoparticles (Ag NPs) by plant extract techniques of green tea and study optical and structural properties. J Nano and Medical Materials. 2023;3(1):145. doi:10.59400/nmm.v3i1.145.
  5. Abbasi R, Shineh G, Mobaraki M, Doughty S, Tayebi L. Structural parameters of nanoparticles affecting their toxicity for biomedical applications: a review. Journal of Nanoparticle Research. 2023;25(3):43.
  6. Mohammed RA, Saleh GM, Mutlak FA. Plant-mediated synthesis of silver nanoparticles and antibacterial activity on implicated biomolecules of green Spinacia oleracea leaf extract. J Nanomed Nanotechnol. 2022;2(4):1-5. doi:10.31031/DNN.2022.02.000545.
  7. Alattar AM, Al-Sharuee IF, Odah JF. Laser fragmentation of green tea-synthesized silver nanoparticles and their blood toxicity: effect of laser wavelength on particle diameters. Journal of Medical Physics. 2024;49(1):95-102.
  8. Gautam S, Das DK, Kaur J, Kumar A, Ubaidullah M, Hasan M, et al. Transition metal-based nanoparticles as potential antimicrobial agents: recent advancements, mechanistic, challenges, and future prospects. Discover Nano. 2023;18(1):1-20.
  9. Mohammed RA, Mutlak FA, Mohammed GM. Nanoparticle preparation and antibacterial-activity analysis using pulsed ablation at 1064 and 532 nm. Nanosistemi, Nanomateriali, Nanotehnologii. 2022;20(3).
  10. Navaf M, Sunooj KV, Aaliya B, Akhila PP, Sudheesh C, Mir SA, et al. Impact of metal and metal oxide nanoparticles on functional and antimicrobial activity of starch nanocomposite film: a review. Measurement: Food. 2023;11.
  11. Mohammed RA, Saleh GM, Mutlak FA. Investigate the structural properties of silver nanoparticles produced by bio production of green Spinacia oleracea leaf extract and their influence on antibacterial activity. Iraqi Journal of Nanotechnology. 2022;3(1):44-49.
  12. [Sarhan AM, Habib AM, Fahmy A, Noor El-Deen TM, Selim A. Effect of nano, bio, chemical fertilization and leaves extract of moringa plant on flowering and chemical constituents of gladiolus plant. Egyptian Journal of Chemistry. 2022;65(7):221-230.
  13. Mohammed RA, Mutlak FA, Saleh GM. Influence of laser ablation wavelengths 355, 532 and 1064 nm on the structural properties of silver nanoparticle size with biological application. JP Journal of Heat and Mass Transfer. 2021;24(2):333-346.