Histological Study of Open Wound Healing with a Light Cure Instrument

Document Type : Original Paper


1 Assistant Professor, Technology Radiology& radiotherapy Dept., Faculty of Paramedical Sciences, Tehran University of Medical Sciences, Tehran, Iran.

2 Associate Professor, Dermatology Dept., Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

3 Assistant Professor of Dermatology, Research Center Professor Torabinejad, Esfahan, Iran.

4 M.Sc. of Biophysics, Science and Research Branch, Islamic Azad University, Tehran, Iran.


Introduction: In the present study, the effects of light cure on wound healing of mice’s skin with complete thickness were studied.
Materials and Methods: Forty male mice of NMRI race were placed randomly into two groups of examination and control. Under aseptic and unconscious conditions, a circular wound of 6 mm width with complete thickness of skin was made on the back of each mice. The surgery day was established as day zero. Blue light of wavelength 420-500 nm from a light cure instrument was shined onto all the mice from the first day. Over the 3rd, 7th, 10th, 15th and 21st days after curing, four kinds of wound and healthy skins were taken from each mice. The practical procedures of general histology were applied on the samples, then slices of 5 micron thickness were taken from them and finally, they were colored with Hematoxline Eosin. The cell population of wound bed including fibroblast cells, macrophages, neutrophils and endothelia of vessels were studied.
Results: The examination group showed significantly increased fibrosis and decreased inflammation (p≤0.05).
Conclusion: Halogen blue light (light cure) causes significant early open wound healing of skin with complete thickness.


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