Capacitive Hyperthermia as an Alternative to Brachytherapy in Treatment of Human Prostate Cancer Cell

Janati Esfahani, Azam; Mahdavi, Seied Rabi; Khoei, Samideh; Bakhshandeh, Mohsen; Rajabi, Ali

doi:10.22038/ijmp.2018.12664

Capacitive Hyperthermia as an Alternative to Brachytherapy in Treatment of Human Prostate Cancer Cell

Document Type : Conference Proceedings

Authors

Azam Janati Esfahani ¹

Seied Rabi Mahdavi ²

Samideh Khoei ³

Mohsen Bakhshandeh ⁴

Ali Rajabi ⁵

¹ Department of Medical Biotechnology, School of Paramedical Sciences, Qazvin University of Medical Sciences, Iran.

² Radiation Biology Research Center, Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

³ Department of Medical Physics, Iran University of Medical Sciences, Tehran, Iran

⁴ Radiology & Radiotherapy department- Para Medical Faculty- Shahid Beheshti University of Medical sciences, Tehran, Iran.

⁵ Radiation Medicine Engineering Department, Shahid Beheshti University, Tehran, Iran

10.22038/ijmp.2018.12664

Abstract

Introduction: The aim of this study was the evaluation of induced DNA damages of human prostate cancer cells, DU-145, treated with a combination of radiofrequency capacitive hyperthermia (HT) and teletherapy (EBRT) compared to a combination of teletherapy with high dose rate brachytherapy (BR).

Materials and Methods: DU-145 cells were cultured as spheroids in 300-micron diameter. Then the following treatments were conducted: a) EBRT at doses of either 2Gy or 4Gy of photon 15MV, b) HT for 30, 60, and 90 minutes’ duration at 43°C from a 13.56MHz radiofrequency capacitive heating device (Celsius TCS), c) BR with Ir-192 seed at doses of either 2Gy or 5.5Gy, d) The mentioned HT followed by EBRT (HT+EBRT) and e) EBRT followed by BR (EBRT+BR). Alkaline comet assay was performed to measure tail moment.
Results: The induced DNA damages of DU-145 cells treated by adding HT to EBRT compared with EBRT alone, showed a significant enhancement; 3.28 and 5.14 times respectively for 30 and 60 minutes HT. By plotting dose-response curves, we could find a range of doses, which create radiobiological iso-effect in HT+EBRT and EBRT+BR treatments.
Conclusion: This study suggests that about DNA damages of DU-145 cells, HT+EBRT could partly be considered as an alternative to EBRT+BR.

Keywords

Prostate cancer

hyperthermia

brachytherapy

teletherapy

comet assay