Document Type : Conference Proceedings
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Department of medical physics, Faculty of Medical, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Inorganic Chemistry, Faculty of Chemistry, Tabriz University, Tabriz, Iran
Department of Radiology, Faculty of Paramedical, Tabriz University of Medical Sciences, Tabriz, Iran.
Introduction: Theranostic nanoparticles, which integrate both diagnostic and therapeutic capabilities into one nanoagent, has great promise to ablate more effective tumoral tissue by optimizing and real-time monitoring treatment interventions, as well as monitoring therapeutic response to corresponding effect. Multifunctional theranostic nanoagent based on graphene quantum dots (GQDs)-coated Bi nanohybrids (GQDs-Bi NPs) was developed and investigate their multimodal imaging and PTT performance.
Materials and Methods: The GQDs-Bi NPs were fabricated by utilizing simple two-step synthesis: GQDs as shell and Bi NPs as core in presence of GQDs. By hemolysis and MTT test, blood compatibility and cellular toxicity of GQDs-Bi NPs were examined. For phantom CT imaging, the GQDs-Bi NPs aqueous solutions at various concentrations of Bi element scanned by a clinical CT scanner (GE HiSpeed) (160 mA, 80,120 and140 kV). The corresponding CT values (HU) were measured by imageJ software. To evaluate the photothermal effect, the GQDs-Bi NPs dispersions at different concentrations of active Bi metal were irradiated with an 808 nm-NIR-laser (1.7 W·cm-2) for 10 min and a thermocouple probe was used to record the temperature of suspensions.
Results: The GQDs-Bi NPs showed satisfactory cytotoxicity and hemolysis behavior. The heavy metal Bi component (Z=83) in the GQDs-Bi NPs produced much higher Hounsfield units (173 HU) than the contrast agents based on small molecules iodine (134 HU), corresponding, 1 mg Bi/ml provided an equivalent X-ray attenuation as dotarem contain of
1.5 mg/mL Iodine. A strong and steady absorbance was found for GQDs-Bi NPs in the whole NIR range, as well as high photo-to-thermal conversion capability and photostability, encouraging a high antitumor PTT efficiency. The GQDs-Bi NPs could successfully kill in vitro MCF-7 and HeLa cancer cells under NIR irradiation with killing effect dependent on dose compared to those received laser only (3.0%).
Conclusion: GQDs-Bi nanotheranostic may become an effective tool for CT imaging-guided therapy for personalized cancer treatment.