Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and Its Definite Dose Reduction Factor

Nadi, Saba; Shabestani Monfared, Ali; Mozdarani, Hossein; Mahmodzade, Aziz; Pouramir, Mahdi

doi:10.22038/ijmp.2018.12890

Effects of Arbutin on Radiation-Induced Micronuclei in Mice Bone Marrow Cells and Its Definite Dose Reduction Factor

Document Type : Conference Proceedings

Authors

Saba Nadi ¹

Ali Shabestani Monfared ²

Hossein Mozdarani ³

Aziz Mahmodzade ⁴

Mahdi Pouramir ⁵

¹ Department of Radiobiology and Radiation Protection, Babol University of Medical Sciences, Babol, Iran

² Cellular & Molecular Biology Research Center, Department of Medical physics, Babol University of Medical Sciences, Babol, Iran

³ Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

⁴ Laboratory of Cytogenetic, Novin Medical Radiation Institute, Tehran, Iran

⁵ Department of Biochemistry, Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran

10.22038/ijmp.2018.12890

Abstract

Introduction:
Interactions of free radicals from ionizing radiation with DNA can induce DNA damage and lead to mutagenesis and carsinogenesis. With respect to radiation damage to human, it is important to protect humans from side effects induced by ionizing radiation. In the present study,the effects of arbutin were investigated by using the micronucleus test for anti- clastogenic activity, to calculate the ratio of polychromatic erythrocyte to polychromatic erythrocyte plus normochromatic erythrocyte (PCE/PCE+NCE) in order to show cell proliferation activity.
Materials and Methods:
Arbutin (50, 100, and 200 mg/kg) was intraperitoneally (ip)administered to NMRI mice two hours before gamma radiation at 2 and 4 gray (Gy). The frequency of micronuclei in 1000 PCEs (MnPCEs) and the ratio of PCE/PCE+NCE were calculated for each sample. Data were statistically evaluated using one-way ANOVA,Tukey HSD test, and t-test.
Results:
The findings indicated that gamma radiation at 2 and 4 Gy extremely increased the frequencies of MnPCE (P<0.001) while reducing PCE/PCE+NCE (P<0.001) compared to the control group. All three doses of arbutin before irradiation significantly reduced the frequencies of MnPCEs and increased the ratio of PCE/PCE+NCE in mice bone marrow compared to the non-drug-treated irradiated control (P<0.001). All three doses of arbutin had no toxicity effect on bone marrow cells. The calculated dose reduction factor (DRF) showed DRF=1.93 for 2Gy and DRF=2.22 for 4 Gy.
Conclusion:
Our results demonstrated that arbutin gives significant protection to rat bone against the clastogenic and cytotoxic effects of gamma irradiation.

Keywords

Gamma rays

Micronucleus tests

Erythrocytes

Bone marrow cell

Arbutin

Radiation protective agents