Study of the Radio-Protective Effects of Henna Mixed With Vinegar on the Skin of Irradiated Rats

Document Type : Original Paper

Authors

1 Department of Medical Physics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

2 Medical Physics Department, Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Department of Pathology, Mashhad University of Medical Sciences, Mashhad, Iran.

4 Kidney Transplantation Complications Research Center, Mashhad University of Medical Sciences, Mashhad, I.R. Iran.

5 Medical Physics Dept, Faculty of Medicine, Mashhad University of Medical Sciences

6 Associate Professor of Medical Physics, Research Center of Medical Physics, Mashhad University of Medical Sciences, Mashhad, Iran

10.22038/ijmp.2022.65850.2128

Abstract

Introduction: Skin damage is one of the most common challenges of radiation therapy. In the present study an attempt was made to evaluate the radio-protective effects of henna mixed with vinegar on the skin.
Material and Methods: The rats were divided into Control (no treatment), R (receiving radiation alone), V (receiving vinegar alone), H (receiving henna mixed with vinegar), R + H (receiving henna mixed with vinegar and then radiation), and R + V (receiving vinegar and then radiation) groups. All rats were euthanized and their skins’ pathological damage and the level of super oxide dismutase (SOD) and Mallon dialdehyde (MDA) were evaluated. The rats were irradiated with 15 Gy (6MV) X-rays.
Results: Increase MDA and decrease SOD were seen in R group. However, these changes were ameliorated in R + H group, although the difference was not significant. Vinegar significantly decreased MDA induced by radiation, but could not increase the SOD level. The rate of skin pathological damage in R group were higher than in Control. However, the level of these lesions was lower in R + H and R + V groups compared to R group. The rate of dermatitis and sweat gland atrophy in R + H and R + V groups were less than R group and similar to the level in the control group.
Conclusion: Henna and vinegar could reduce the skin injuries induced by radiation. Regarding pathological injuries, the effects of vinegar alone was more than henna, but regarding antioxidant activity, the protective effect of henna mixed with vinegar was better than vinegar alone.

Keywords

Main Subjects

References

  1. Barton MB, Frommer M, Shafiq J. Role of radiotherapy in cancer control in low-income and middle-income countries. The lancet oncology. 2006;7(7):584-95.
  2. Wallace SS. Enzymatic processing of radiation-induced free radical damage in DNA. Radiation research. 1998;150(5s):S60-S79.
  3. Barazzuol L, Coppes RP, van Luijk P. Prevention and treatment of radiotherapy‐induced side effects. Molecular Oncology. 2020.
  4. Lai-Cheong JE, McGrath JA. Structure and function of skin, hair and nails. Medicine. 2017;45(6):347-51.
  5. Xiao Y, Mo W, Jia H. Ionizing radiation induces cutaneous lipid remolding and skin adipocytes confer protection against radiation-induced skin injury. Journal of dermatological science. 2020;97(2):152-60.
  6. Wan Y, Tu W, Tang Y. Prevention and Treatment for Radiation-Induced Skin Injury during Radiotherapy. Radiation Medicine and Protection. 2020.
  7. Patyar RR, Patyar S. Role of drugs in the prevention and amelioration of radiation induced toxic effects. European journal of pharmacology. 2018;819:207-16.
  8. Chandrasekharan DK, Khanna PK, Nair CKK. Cellular radioprotecting potential of glyzyrrhizic acid, silver nanoparticle and their complex. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2011;723(1):51-7.
  9. Basaga H, Tekkaya C, Acikel F. Antioxidative and free radical scavenging properties of rosemary extract. LWT-Food Science and Technology. 1997;30(1):105-8.
  10. Mun G-I, Kim S, Choi E. Pharmacology of natural radioprotectors. Archives of Pharmacal Research. 2018 2018/11/01;41(11):1033-50.
  11. Mikhaeil BR, Badria FA, Maatooq GT. Antioxidant and immunomodulatory constituents of henna leaves. Zeitschrift für Naturforschung C. 2004;59(7-8):468-76.
  12. Hosein HKM, Zinab D. Phenolic compounds and antioxidant activity of henna leaves extracts (Lawsonia inermis). World Journal of Dairy & Food Sciences. 2007;2(1):38-41.
  13. Al-Damegh MA. Evaluation of the antioxidant activity effect of henna (Lawsonia inermis Linn.) leaves and or vitamin C in rats. Life Sci J. 2014;11:234-41.
  14. Sakarkar DM, Sakarkar UM, Shrikhande VN, Vyas JV, Mandavgade S, Jaiswal SB, et al. Wound healing properties of Henna leaves.
  15. Al-Rubiay KK, Jaber NN, Al-Mhaawe BH, Alrubaiy LK. Antimicrobial efficacy of henna extracts. Oman medical journal. 2008 Oct;23(4):253.
  16. Hadisi Z, Nourmohammadi J, Nassiri SM. The antibacterial and anti-inflammatory investigation of Lawsonia Inermis-gelatin-starch nano-fibrous dressing in burn wound. International journal of biological macromolecules. 2018;107:2008-19.
  17. Singh DK, Luqman S. Lawsonia inermis (L.): a perspective on anticancer potential of mehndi/henna. Biomedical Research and Therapy. 2014;1(04):112-20.
  18. J Kapadia G, Subba Rao G, Sridhar R, Ichiishi E, Takasaki M, Suzuki N, et al. Chemoprevention of skin cancer: effect of Lawsonia inermis L.(Henna) leaf powder and its pigment artifact, lawsone in the Epstein-Barr virus early antigen activation assay and in two-stage mouse skin carcinogenesis models. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents). 2013 Dec 1;13(10):1500-7.
  19. Nakashima S, Oda Y, Nakamura S, Liu J, Onishi K, Kawabata M, et al. Inhibitors of melanogenesis in B16 melanoma 4A5 cells from flower buds of Lawsonia inermis (Henna). Bioorganic & Medicinal Chemistry Letters. 2015 Jul 1;25(13):2702-6.
  20. Yaralizadeh M, Abedi P, Namjoyan F, Fatahinia M, Chegini SN. A comparison of the effects of Lawsonia inermis (Iranian henna) and clotrimazole on Candida albicans in rats. Journal de Mycologie Médicale. 2018 Sep 1;28(3):419-23.
  21. Jridi M, Sellimi S, Lassoued KB, et al. Wound healing activity of cuttlefish gelatin gels and films enriched by henna (Lawsonia inermis) extract. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2017;512:71-9.
  22. Sibin Melo KC, Correia MH, Svidzinski TI, Hernandes L. Exocellular extract of Fusarium oxysporum, fungus free, is able to permeate and act selectively in skin. Apmis. 2018 May;126(5):418-27.
  23. Beyer Jr WF, Fridovich I. Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Analytical biochemistry. 1987;161(2):559-66.
  24. Madesh M, Balasubramanian K. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian journal of biochemistry & biophysics. 1998;35(3):184-8.
  25. Salvo N, Barnes E, Van Draanen J, Stacey E, Mitera G, Breen D, Giotis A, et al. Prophylaxis and management of acute radiation-induced skin reactions: a systematic review of the literature. Current oncology. 2010 Aug;17(4):94.
  26. Connell PP, Hellman S. Advances in radiotherapy and implications for the next century: a historical perspective. Cancer research. 2009;69(2):383-92.
  27. Yamini K, Gopal V. Natural radioprotective agents against ionizing radiation-an overview. International Journal of PharmTech Research. 2010;2(2):1421-6.
  28. Weiss JF, Landauer MR. Radioprotection by Antioxidants a. Annals of the New York Academy of Sciences. 2000;899(1):44-60.
  29. Samarth RM, Panwar M, Kumar M, Soni A, Kumar M, Kumar A. Evaluation of antioxidant and radical-scavenging activities of certain radioprotective plant extracts. Food chemistry. 2008 Jan 15;106(2):868-73.
  30. Hsouna AB, Trigui M, Culioli G, Blache Y, Jaoua S. Antioxidant constituents from Lawsonia inermis leaves: Isolation, structure elucidation and antioxidative capacity. Food Chemistry. 2011 Mar 1;125(1):193-200.
  31. Duan W, Xia T, Zhang B, Li S, Zhang C, Zhao C, et al. Changes of physicochemical, bioactive compounds and antioxidant capacity during the brewing process of Zhenjiang aromatic vinegar. Molecules. 2019 Oct 31;24(21):3935.
  32. da Silva Santin M, Koehler J, Rocha DM, Dos Reis CA, Omar NF, Fidler Y, et al. Initial damage produced by a single 15-Gy x-ray irradiation to the rat calvaria skin. European Radiology Experimental. 2020 Dec;4:1-0.
  33. Karslioğlu I, Ertekin MV, Taysi S, et al. Radioprotective effects of melatonin on radiation-induced cataract. Journal of radiation research. 2005;46(2):277-82.
  34. Lu X, Wang Y, Zhang Z. Radioprotective activity of betalains from red beets in mice exposed to gamma irradiation. European journal of pharmacology. 2009;615(1-3):223-7.

 

 

 

 

 

Iranian Journal of Medical Physics
Volume 21, Issue 4 - Serial Number 4
July and August 2024
Pages 249-257

Files

History

  • Receive Date: 30 May 2022
  • Revise Date: 30 September 2024
  • Accept Date: 08 October 2022

Share

How to cite

Statistics