Preliminary Results of Treating Cancerous Cells of Lung (QU-DB) by Hyperthermia using Diode Laser and Gold Coated Fe3O4/SiO2 Nano-Shells: An in-Vitro Assay

Document Type: Original Paper

Authors

1 Laser and Nano-biophotonics Lab., Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran. Laser, optics and photonics research center, Tehran, Iran.

2 Laser and Nano-biophotonics Lab., Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.

3 -Laser and Nano-biophotonics Lab., Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.

Abstract

Introduction
In this study, we describe the results of controlled synthesis and application of gold coated Fe3O4/SiO2 nano-shells combined with the optical property of gold for enhancement of selective photothermal interaction with cancerous cells based on the surface plasmon resonance (SPR) Phenomena.
Materials and Methods
Magnetite Nano-Particles (MNPs) were prepared by means of co-precipitation. MNPs were modified with a thin layer of Silica using the Stober method. The amino-modified Fe3O4/SiO2 nano-shells were covered with gold colloids as a self-assembeled process. In-vitro assays were performed to determine the effect of apoptosis of the cells based on the cells morphological changes.
Results
The biologically inert nano-shells (85 nm) with a Magnetite/Silica core and a gold shell were optically activated. A successful laser-hyperthermia based on the thermal effect of surface plasmon resonance was performed using different gold concentrations. The thermal profile effects of laser power are presented as ideal cases of nanoshell-assisted photo-thermal therapy. The thermally-induced cell death has been shown to be dependent on NPs concentration and laser power density. The power densities of 157 and 184 W/cm2 caused complete cell death at the focal point of the laser beam.Cell damage was reduced by decreasing the power density of laser. Also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation.
Conclusion
An optimized laser-(SPR) hyperthermia was obtained using a concentration of gold coated Fe3O4/SiO2 nano-shells concentration=0.1 mg/ml at intensity=157 W/cm2 at 60s.

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Volume 9, Issue 4
November and December 2012
Pages 253-263
  • Receive Date: 18 July 2012
  • Revise Date: 16 March 2013
  • Accept Date: 16 December 2012